Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-05-18
1998-03-03
McElwain, Elizabeth
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 435 691, 4351723, 435410, 536 2371, A01H 500, C12N 504, C12N 1532, C12N 1582
Patent
active
057237565
ABSTRACT:
Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession numbers 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.
REFERENCES:
Chemical Abstracts, vol. 109, No. 5, Aug. 1, 1988, "Insect Resistance in Transgenic Plants Expressing Bacillus Thuringiensis Toxin Genes", p. 176, Abstract No. 33142v.
Chemical Abstracts, vol. 109, No. 17, Oct. 24, 1988, "Engineering of Insect Resistant Plants Using a B. thuringiensis Gene", pp. 211-212, Abstract No. 143900y.
Microbiological Reviews, vol. 53, No. 2, Jun. 1989, pp. 242-255, "Insecticidal Crystal Proteins of Bacillus thuringiensis", Herman Hofte, et al.
Nucleic Acids Research, vol. 18, No. 5, Dec. 19, 1989, "Nucleotide Sequence of a Coleopteran-Active Toxin Gene from a New Isolate of Bacillus thuringiensis susp. tolworthi", Aug. Sick, et al.
Journal of Biochemistry, vol. 270, pp. 133-136, "The Construction of Bacillus thurigiensis Strains Expressing Novel Entromicidal .delta.-Endotoxin Combinations", Neil Crickmore, et al.
Proc. Natl. Acad. Sci., vol. 88, pp. 3324-3328, Apr. 1991, "Modification of Coding Sequence Enhances Plant Expression of Insect Control Protein Genes", Frederick J. Perlak, et al.
MacIntosh et al. (1990) J. of Invertebrate Pathology 56: 258-266.
Lambert Bart
Peferoen Marnix
Van Audenhove Katrien
McElwain Elizabeth
Plant Genetic Systems N.V.
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