Bacillus thuringiensis strains and their genes encoding insectic

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Bacteria or actinomycetales

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424405, 43525231, 435832, A01N 6300, A01N 2500, C12N 100, C12N 120

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active

058372377

ABSTRACT:
Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession numbers 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.

REFERENCES:
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patent: 5659123 (1997-08-01), Van Rie et al.
Chemical Abstracts, vol. 109, No. 5, Aug. 1, 1988, "Insect Resistance in Transgenic Plants Expressing Bacillus Thuringiensis Toxin Genes", p. 176, Abstract No. 33142v.
Chemical Abstracts, vol. 109, No. 17, Oct. 24, 1988, "Engineering of Insect Resistant Plants Using a B. thuringiensis Gene", pp. 211-212, Abstract No. 143900y.
Microbiological Reviews, vol. 53, No. 2, Jun. 1989, pp. 242-255, "Insecticidal Crystal Proteins of Bacillus thuringiensis", Herman Hofte, et al.
Nucleic Acids Research, vol. 18, No. 5, Dec. 19, 1989, "Nucleotide Sequence of a Coleopteran-Active Toxin Gene from a New Isolate of Bacillus thuringiensis susp. tolworthi", Aug. Sick, et al.
Journal of Biochemistry, vol. 270, pp. 133-136, "The Construction of Bacillus thurigiensis Strains Expressing Novel Entromicidal .delta.-Endotoxin Combinations", Neil Crickmore, et al.
Proc. Natl. Acad. Sci., vol. 88, pp. 3324-3328, Apr. 1991, "Modification of Coding Sequence Enhances Plant Expression of Insect Control Protein Genes", Frederick J. Perlak, et al.
J. of Invertebrate Pathology, MacIntosh et al., vol. 56, 1990, pp. 258-266.

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