Bacillus thuringiensis mutants which produce high yields of crys

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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424 932, 424 93461, 424DIG8, 435832, C12N 120

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active

056020325

ABSTRACT:
The invention relates to a mutant of Bacillus thuringiensis which produces a larger amount of crystal delta-endotoxin with a greater pesticidal activity as compared to the corresponding parental strain. The mutant may also have a larger crystal size as compared to the corresponding parental strain. The crystal delta-endotoxin produced by the mutant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parental Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to a method for producing such a mutant, compositions comprising such a mutant as well as methods for controlling a pest(s) using these compositions.

REFERENCES:
patent: 4766203 (1988-08-01), Krieg et al.
patent: 4990332 (1991-02-01), Payne et al.
patent: 5279962 (1994-01-01), Gurtler et al.
Wakisaka et al., Applied & Environ. Microbiol., 43(6): 1498-1500 (Jun. 1982).
Hofte et al, Microbiological Reviews, 53(2): 242-255 (1989).
Johnson et al, Applied & Environ. Microbiol., 42(2): 385-387 (1981).
Krieg et al, Journal of Applied Entomology, 96:500-508 (1983).
Herrnstadt et al., Bio/Technology, 4:305-308 (1986).
C. C. Payne et al; British Crop Protection Conference-Pests & Diseases, pp. 231-238 (1984).
Nishiitsutsuji-Uwo et al, J. Invert. Pathol., 25:355-361 (1975).
Johnson et al, Can. J. Microbiol., 26:486-491 (1980).

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