Bacillus thuringiensis isolates with broad spectrum activity

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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4353201, 536 2371, A01H 500, A01H 510, C12N 1532, C12N 1582

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active

059861770

ABSTRACT:
A broad spectrum Bacillus strain is provided which displays activity against at least two orders of insects. Novel proteins active against corn rootworm and nematodes and methods of isolation are additionally provided. Also provided is a purified crystal protein complex isolated from a Bacillus strain that demonstrates insecticidal properties. In addition, the nucleotide and amino acid sequence of the Cry1I gene isolated from this strain is provided.

REFERENCES:
Hofte H, et al. "Insecticidal crystal proteins of Bacillus thuringiensis." Microbiol. Rev. 53: 242-255, 1989.
Schnepf HE, et al. "Bacillus thuringiensis toxins: regulation, activities, and structural diversity." Curr. Opinion Biotech. 6: 305-312, 1995.
Shin et al. "Distribution of Cry V-type Insecticidal Protein Genes in Bacillus Thuringiensis and Cloning of Cry V-type Genes from Bacillus Thuringiensis subsp. Kurstaki and Bacillus Thuringiensis subsp. Entomocidus", Applied and Environmental Microbiology 61:2402-2407 (1995).
Chambers et al. "Isolation and Characteriziation of a Novel Insecticidal Crystal Protein Gene from Bacillus Thuringiensis subsp. Aizawai", Journal of Bacteriology 173:3966-3976 (1991).
Lee et al. "Diversity of Protein Inclusion Bodies and Identification of Mosquitocidal Protein in Bacillus Thuringiensis subsp. israelensis", Biochemical and Biophysical Research Communications 126:953-960 (1985).

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