Bacillus thuringiensis isolates, toxins, and genes selectively a

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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435419, 800302, 536 237, A01N 6300, C12N 504, C12N 510

Patent

active

060715111

ABSTRACT:
The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests, e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests. In preferred embodiments, a gene from PS140E2 is used to transform plants so that the transformed plants are resistant to flea beetles of the genus Phyllotreta.

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