Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2000-03-09
2003-06-03
Bugaisky, Gabrielle (Department: 1646)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C514S002600, C530S350000
Reexamination Certificate
active
06573240
ABSTRACT:
BACKGROUND OF THE INVENTION
The most widely used microbial pesticides are derived from the bacterium
Bacillus thuringiensis.
This bacterial agent is used to control a wide range of leaf-eating caterpillars and beetles, as well as mosquitos.
Bacillus thuringiensis
produces a proteinaceous parasporal body or crystal which is toxic upon ingestion by a susceptible insect host. For example,
B. thuringiensis
subsp.
kurstaki
HD-1 produces a crystal inclusion consisting of a biotoxin called a delta toxin which is toxic to the larvae of a number of lepidopteran insects. The cloning, sequencing, and expression of this
B.t.
crystal protein gene in
Escherichia coli
has been described in the published literature (Schnepf, H. E. and Whitely, H. R. [1981] Proc. Natl. Acad. Sci. USA 78:2893-2897; Schnepf et al.). U.S. Pat. Nos. 4,448,885 and 4,467,036 both disclose the expression of
B.t.
crystal protein in
E. coli.
BRIEF SUMMARY OF THE INVENTION
The subject invention concerns a novel
Bacillus thuringiensis
isolate designated
B.t.
PS81I which has activity against all lepidopteran pests tested.
Also disclosed and claimed are novel toxin genes which express toxins toxic to lepidopteran insects. These toxin genes can be transferred to suitable hosts via a plasmid vector.
Specifically, the invention comprises the novel
B.t.
isolate denoted
B.t.
PS81I, mutants thereof, and novel &dgr;-endotoxin genes derived from this
B.t.
isolate which encode proteins which are active against lepidopteran pests.
BRIEF DESCRIPTION OF THE SEQUENCES
SEQ ID NO:1 is the nucleotide sequence of the novel
B.t.
toxin gene PS81IA2.
SEQ ID NO:2 is the amino acid sequence of the novel
B.t.
toxin PS81IA2.
SEQ ID NO:3 is the nucleotide sequence of the novel
B.t.
toxin gene PS81IB.
SEQ ID NO:4 is the amino acid sequence of the novel
B.t.
toxin PS81IB.
SEQ ID NO:5 is the nucleotide sequence of the novel
B.t.
toxin gene PS81IB2.
SEQ ID NO:6 is the amino acid sequence of the novel
B.t.
toxin PS81IB2.
SEQ ID NO:7 is the nucleotide sequence of the novel
B.t.
toxin gene PS81IA.
SEQ ID NO:8 is the amino acid sequence of the novel
B.t.
toxin PS81IA.
REFERENCES:
patent: 5126133 (1992-06-01), Payne et al.
patent: 5164180 (1992-11-01), Payne et al.
patent: 5206166 (1993-04-01), Payne et al.
patent: 5246852 (1993-09-01), Payne et al.
patent: 5407825 (1995-04-01), Payne et al.
patent: 5691308 (1997-11-01), Payne et al.
patent: 0632335 (1993-05-01), None
patent: 0400246 (1990-05-01), None
Honée et al. (1988), “Nucleotide sequence of crystal protein gene isolated fromB. thuringiensissubspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species,”Nucleic Acids Research16(13):6240.
Sanchis et al. (1989), “Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active &dgr;-endotoxin gene ofBacillus thuringiensis aizawai7.29,”Molecular Microbiology3(2):229-238.
Hofte et al. (1990), “Nucleotide sequence and deduced amino acid sequence of a new Lepidoptera-specific crystal protein gene fromBacillus thuringiensis, ”Nucleic Acids Research18(18):5545.
Chambers et al. (1991), “Isolation and Characterization of a Novel Insecticidal Crystal Protein Gene fromBacillus thuringiensissubsp. aizawai,”Journal of Bacteriology173(13):3966-3976.
Payne Jewel
Sick August J.
Bugaisky Gabrielle
Mycogen Corporation
Saliwanchik Lloyd & Saliwanchik
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