Bacillus thuringiensis gene encoding a toxin active against inse

Details Bacillus thuringiensis gene encoding a toxin active against inse Bacillus thuringiensis gene encoding a toxin active against inse

1994-06-10

1996-06-25

Chereskin, Che S.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

536 2371, 4352523, 43525231, 43525234, 4352351, 435 691, 4353201, 424 932, 800DIG56, 514 12, 530350, C07K 14325, A01H 400, C12N 1532, C12N 1582

Patent

active

055301959

ABSTRACT:
The present invention is drawn to a novel toxin gene purified and isolated from Bacillus thuringiensis var kurstaki and given the designation CryIE(c). The novel toxin gene encodes a protein of approximately 130 kDa in size and is active against Lepidopteran insects. Also included in the invention are the proteins encoded by CryIE(c). Further disclosed are recombinant genes and proteins derived from CryIE(c). Also provided are biologically pure bacterial strains transformed with the CryIE(c) gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention are plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.

REFERENCES:
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patent: 5143905 (1992-09-01), Vasubramanian et al.
Ge et al., "Location of the Bombyx mori specificity domain on a Bacillus thuringiensis .delta.-endotoxin protein", Proc. Natl. Acad. Sci. USA, 86:4037-4041 (1989).
Feitelson, "GenBank/EMBL Sequence Database, Accession No. M73253, cryIE(b) gene" (1991).
Hofte et al., "Insecticidal Crystal Proteins of Bacillus thuringiensis", Microbiological Reviews, 53(2):242-255 (1989).
Li et al., "Crystal structure of insecticidal .delta.-endotoxin from Bacillus thuringiensis at 2.5 .ANG. resolution", Nature, 353:815-821 (1991).
Schnepf et al., "Specificity-determining Regions of a Lepidopteran.varies.specific Insecticidal Protein Produced by Bacillus thuringiensis", The Journal of Biological Chemistry, 265(34):20923-20930 (1990).
Visser et al., "A Novel Bacillus thuringiensis Gene Encoding a Spodoptera exigua-Specific Crystal Protein", Journal of Bacteriology, 172(12):6783-6788 (1990).
Schnepf, et al (1990) Journal Biol. Chem. 265 (34):20923-20930.
Murray, et al (1991) Plant Molecular Biology 16: 1035, Abstract.

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