Bacillus thuringiensis cryIIIc(B) toxin gene and protein toxic t

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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424 93L, 435 6, 435 691, 4352523, 4353201, 536 221, 536 231, 536 232, 536 237, 536 2371, C12N 120, A01N 6300, C12Q 168, C12P 2106

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052643646

ABSTRACT:
A Bacillus thuringiensis strain isolate, designated EG5144, exhibits insecticidal activity against coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica. A novel toxin gene in B.t. strain EG5144 produces an irregularly shaped insecticidal crystal protein of approximately 70 kDa that is toxic to coleopteran insects. The cryIII-type gene (SEQ ID NO:1), designated as the cryIIIC(b) gene, has a nucleotide base sequence illustrated in FIG. 1.

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