Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-03-01
2009-08-11
Benzion, Gary (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100
Reexamination Certificate
active
07572587
ABSTRACT:
Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
REFERENCES:
patent: 4547360 (1985-10-01), Perlberg
patent: 5173409 (1992-12-01), English
patent: 5187091 (1993-02-01), Donovan et al.
patent: 5264364 (1993-11-01), Donovan et al.
patent: 5276269 (1994-01-01), Raikhel
patent: 5338544 (1994-08-01), Donovan
patent: 5449681 (1995-09-01), Wickiser
patent: 5482852 (1996-01-01), Yoder et al.
patent: 5508468 (1996-04-01), Lundquist et al.
patent: 5550318 (1996-08-01), Adams et al.
patent: 6537756 (2003-03-01), Rupar et al.
patent: 6593114 (2003-07-01), Kunsch et al.
patent: WO9116433 (1991-10-01), None
patent: WO94/13785 (1994-06-01), None
patent: WO94/16079 (1994-07-01), None
patent: WO95/02693 (1995-01-01), None
patent: WO9618302 (1996-06-01), None
patent: WO98/13497 (1998-04-01), None
Baum and Malvar, “Regulation of insecticidal crystal protein production inBacillus thuringiensis,” Molecular Microbiology, 18(1):1-12 (1995).
Chilcott and Ellar, “Comparative Toxicity ofBacillus thuringiensisvar.israelensisCrystal Proteins in vivo and in vitro,”J. of General Microbiology, 134:2551-2558 (1988).
Dhir et al., “Regeneration of transformed shoots from electroporated soybean (Glycine max(L.) Merr.) protoplasts,”Plant Cell Reports, 10:97-101 (1991).
Donovan et al., “Characterization of Two Genes EncodingBacillus thuringiensisInsecticidal Crystal Proteins Toxic toColeopteraSpecies,”Applied and Environmental Microbiology, 58(12):3921-3927 (Dec. 1992).
Earp and Ellar, “Bacillus thuringiensisvar.morrisonistrain PG14: nucleotide sequence of a gene encoding a 27kDa crystal protein,”Nucleic Acids Research, 15(8):3619 (1987).
Knowles et al., “A broad-spectrum cytolytic toxin fromBacillus thuringiensisvar.kyushuensis,” Proc. R. Soc. Lond. B, 248:1-7 (1992).
Koni and Ellar, “Cloning and Characterization of a NovelBacillus thuringiensisCytolytic Delta-Endotoxin,”J. Mol. Biol., 229:319-327 (1993).
Ward et al., “Cloning and expression inEscherichia coliof the insecticidal δ-endotoxin gene ofBacillus thuringiensisvar.israelensis,” FEBS, 175(2):377-382 (Oct. 1984).
Ward and Ellar, “Bacillus thuringiensisvar.israelensisδ-Endotoxin Nucleotide Sequence and Characterization of the Transcripts inBacillus thuringiensisandEscherichia coli,” J. Mol. Biol., 191:1-11 (1986).
Ely, S.: “The engineering of plants to expressBacillus thuringiensisdelta-endotoxins”; Entwistle, E. et al. (Eds.): ‘Bacillus thuringiensis, an Environmental Biopesticide: Theory and Practice’ 1993, GB, Chichester, Wiley & Sons, pp. 105-124, XP002054693.
Donovan William P.
Rupar Mark J.
Slaney Annette C.
Tan Yuping
Ball, Esq. Timothy K.
Benzion Gary
Howrey LLP
Monsanto Technology LLC
Wilder Cynthia B
LandOfFree
Bacillus thuringiensis CryET29 compositions toxic to... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bacillus thuringiensis CryET29 compositions toxic to..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacillus thuringiensis CryET29 compositions toxic to... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4107307