Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1993-03-17
1994-10-18
Chereskin, Che S.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
536 2371, 4353201, 4352523, 43525231, 435 691, 424 93461, C12N 121, C12N 122, C12N 1532, A01N 6300
Patent
active
053566238
ABSTRACT:
A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against lepidopteran insects. A purified and isolated novel cryET1 toxin gene product from B.t. strain EG5092 exhibits specific insecticidal activity against Plutella xylostella (Diamondback moth). The cryET1 gene (SEQ ID NO:1) has a nucleotide base sequence illustrated in FIG. 1 and produces a CryET1 gene product (SEQ ID NO:2) having a deduced amino acid sequence illustrated in FIG. 1.
REFERENCES:
patent: 5064648 (1991-11-01), Hickle et al.
Gleave et al., J. Gen. Microbiol. 138: 55-62 (1992) "Identification of an insecticidal crystal protein from Bacillus thuringiensis DSIR517 with significant sequence differences from previously described toxins".
Smulevitch et al., FEBS Lett 293:25-28(1991) "Nucleotide sequence of a novel .delta.-endotoxin gene cryIg of Bacillus thuringiensis ssp. galleriae".
Chambers et al., J. Bacteriol. 173: 3966-3976 (1991) "Isolation and Characterization of a Novel Insecticidal Crystal Protein Gene from Bacillus thuringiensis subsp. aizawai".
Perlak et al., Proc. Natl. Acad. Sci. USA 88: 3324-3328 (1991) "Modification of the coding sequence enhances plant expression of insect control protein genes".
Von Tersch et al., Appl. Environ. Microbiology 57: 349-358 (1991) "Insecticidal Toxins from Bacillus thuringiensis subsp. kenvae: Gene Cloning and Characterization and Comparison with B. thuringiensis subsp. kurstaki CryIA(c) Toxins".
Visser et al., J. Bacteriol. 172: 6783-6788 (1990) "A Novel Bacillus thuringiensis Gene Encoding a Spodoptera exigua-Specific Crystal Protein".
Hodgman et al., J. DNA Sequencing and Mapping 1: 97-106 (1990) "Models for the structure and function of the Bacillus thuringiensis .delta.-endotoxins determined by compilational analysis".
Hofte et al., Microbiol. Rev. 53: 242-255 (1989) "Insecticidal Crystal Proteins of Bacillus thuringiensis".
Ge et al., Proc. Natl. Acad. Sci. USA 86: 4037-4041 (1989) "Location of the Bombyx mori specificity domain on a Bacillus thuringiensis .delta.-endotoxin protein".
Sanchis et al., Molecular Microbiol. 3: 229-238 (1989) "Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active .delta.-endotoxin gene of Bacillus thuringiensis aizawai 7.29".
Masson, et al., Nucl. Acids Res. 17: 446 (1989) "Nucleotide sequence of a gene cloned from Bacillus thuringiensis subspecies entomocidus coding for an insecticidal protein toxic for Bombyx mori".
Haider et al., Nucl. Acids Res. 16: 10927 (1988) "Nucleotide sequence of a Bacillus thuringiensis aizawai IC1 entomocidal crystal protein gene".
Honee et al., Nucl. Acids Res. 16: 6240 (1988) "Nucleotide sequence of crystal protein gene isolated from B. thuringiensis subspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species".
Brizzard et al., Nucl. Acids Res. 16:2723-2724 (1988) "Nucleotide sequence of an additional crystal protein gene cloned from Bacillus thuringiensis".
Shimizu et al., Agric. Biol. Chem. 52: 1565-1573 (1988) "Cloning and Expression in Escherichia coli of the 135-kDa Insecticidal Protein Gene from Bacillus thuringiensis subsp. aizawai IPL7".
Visser et al., Mol. Gen. Genet. 212: 219-224 (1988) "Genes from Bacillus thuringiensis entomocidus 60.5 coding for insect-specific crystal proteins".
Donovan et al., J. Biol. Chem. 263: 561-567 (1988) "Amino Acid Sequence and Entomocidal Activity of the P2 Crystal Protein".
Fischoff et al., Bio/Technology 5: 807-813 (1987) "Insect Tolerant Transgenic Tomato Plants".
Ward et al., Nucl. Acids Res. 15: 7195 (1987) "Nucleotide sequence of a Bacillus thuringiensis var. israelensis gene encoding a 130 kDa delta-endotoxin".
Herrnstadt et al., Gene 57: 37-46 (1987) "Nucleotide sequence and deduced amino acid sequence of a coleopteran-active delta-endotoxin gene from Bacillus thuringiensis subsp. san diego".
Oeda et al., Gene 53: 113-119 (1987) "Nucleotide sequence of the insecticidal protein gene of Bacillus thuringiensis strain aizawai IPL7 and its high-level expression in Escherichia coli".
Hefford et al., J. Biotechnology 6: 307-322 (1987) "Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp. kurstaki NRD-12".
Kondo et al., Agric. Biol. Chem. 51: 455-463 (1987) "Cloning and Nucleotide Sequencing of Two Insecticidal .delta.-Endotoxin Genes from Bacillus thuringiensis var. kurstaki HD-1 DNA".
Geiser et al., Gene 48: 109-118 (1986) "The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhdl gene of subsp. kurstaki HD1".
Wabiko et al., DNA 5:305-314 (1986) "Bacillus thuringiensis Entomocidal Protoxin Gene Sequence and Gene Product Analysis".
Hofte et al., Eur. J. Biochem. 161:273-280 (1986) "Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715".
Schnepf et al., J. Biol. Chem. 260: 6264-6272 (1985) "The Amino Acid Sequence of a Crystal Protein from Bacillus thuringiensis Deduced from the DNA Base Sequence*".
Adang et al., Gene 36:289-300 (1985) "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta".
Shibano et al., Gene 34:243-251 (1985) "Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein".
Carlton et al., "Plasmids and Delta-Endotoxin Production in Different Subspecies of Bacillus thuringiensis", pp. 246-252, in Molecular Biology of Microbial Differentiation, J. A. Hoch and P. Setlow, ed., American Society for Microbiology, Washington, D.C. (1985).
King et al., "Heliothis Virescens", in Handbook of Insect Rearing, Vol. II, P. Singh and R. F. Moore (eds.), pp. 323-328, Elsevier Science, Amsterdam, (1985).
Fitz-James et al., J. Invertebr. Pathol. 43: 47-58 (1984) "A Surface Net on Parasporal Inclusions of Bacillus thuringiensis".
Kaiser et al., Science 223: 249-255 (1984) "Amphiphilic Secondary Structure: Design of Peptide Hormones".
Gonzalez Jose M.
Von Tersch Michael A.
Chereskin Che S.
Ecogen Inc.
Egolf Christopher
Nadel Alan S.
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