Bacillus thuringiensis Cry1C compositions toxic to lepidopteran

Details Bacillus thuringiensis Cry1C compositions toxic to lepidopteran Bacillus thuringiensis Cry1C compositions toxic to lepidopteran

1996-11-27

1999-08-24

Carlson, Karen Cochrane

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

435 691, 43525231, 4353201, 435410, 536 2371, 536 241, 530350, 514 2, 514 12, A01H 104, C12P 2106, C07H 1700, C07K 1400

Patent

active

059426640

ABSTRACT:
Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

REFERENCES:
Chen et al (1995)J. Biol.Chem. 270:6412-6419. "Mutations in Domain I of Bacillus thuringiensis .delta.-endotoxin CryIAb reduce the Irreversible Binding of Toxin Tomanduca . . . ".
Kalman et al (1993) Appl. Environ. Micro 59:1131-1137. "Cloning of a Novel Cry1C-Type Gene from a Strain of Bacillus thuringiensis Subsp. Galleriae".
Angsuthanasombat et al. (1993) FEMS MicroLett 111:255-262. "Effects on Toxicity of Eliminating a Clearage site in a Predicted Interhelical Loop in Bacillus thuringiensis . . . ".
Aronson et al (1995) J Bact. 177:4059-4065 "Mutagenesis of Specificity and Toxicity . . . ".
Li et al (1991) Nature 353:815-821. "Crystal Structure of Insecticidal .delta.-endotoxin . . . ".

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