Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Bacteria or actinomycetales
Patent
1995-03-31
1998-06-02
Wax, Robert A.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Bacteria or actinomycetales
4352523, 43525231, 435219, 435212, 435220, 4353201, 530350, 530300, 536 231, 536 232, 536 237, 536 2371, A01N 6300, C12N 120
Patent
active
057595380
ABSTRACT:
The present invention is based on the discovery, isolation, sequencing and characterization of two protease genes present in Bacillus thuringiensis (B.t.). One is an alkaline protease gene generally designated "apr. " The other is a neutral protease gene generally designated "npr." The invention also includes genetically disabling the apr and the npr genes to reduce proteolytic activity of the proteases encoded by the genes, and using the disabled genes in insecticidal B.t. constructs along with one or more B.t. protein toxin genes. These resulting B.t. constructs are useful for the enhanced production of B.t. toxin protein. The B.t. toxin protein recovered from the fermentation production of these B.t. constructs also exhibits improved stability with respect to its insecticidal activity.
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Donovan William P.
Tan Yuping
Monsanto Company
Nashed Nashaat T.
Wax Robert A.
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