Bacillus thuringiensis .alpha.-endotoxin fragments

Details Bacillus thuringiensis .alpha.-endotoxin fragments Bacillus thuringiensis .alpha.-endotoxin fragments

1995-06-07

1998-01-20

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525131, 43525233, 536 2371, C12P 2100, C12N 120, C07H 2104

Patent

active

057100209

ABSTRACT:
The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3'-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3'-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.

REFERENCES:
patent: 4448885 (1984-05-01), Schnepf et al.
Huber, H.E., P. Luthy (1981) "Bacillus thuringiensis Delta-Endotoxins: Composition and Activation" Pathogenesis of invertebrate Microbial Diseases , pp. 209-234.
Chang, Shing (1983) "Insecticidal proteins from Bacillus thuringiensis" Trends in BioTech 1(4):100-101.
Wong, H.C.W. et al. (1983) "Transcriptional and Translational Start Sites for the Bacillus thuringiensis Crystal Protein Gene" The Journal of Biological Chemistry 258;1960-1967.
Whiteley, H.R. et al. (1982) "Cloning the Crystal Protein Gene of B. thuringiensis in E. coli" Molecular Cloning and Gene Regulation in Bacilli, pp. 131-144.

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