Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-07-18
1992-01-28
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 694, 4351723, 43525231, 935 60, 935 74, C12N 1500, C12N 121, C12N 1575, C12P 2100
Patent
active
050843834
ABSTRACT:
Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain.
The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium.
For example, the accumulation level of human growth hormone secreted by a B. subtilis MT-430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activity-deficient strain.
REFERENCES:
patent: 4711843 (1987-12-01), Chang
patent: 4801537 (1989-01-01), Nagarajan et al.
patent: 4824782 (1989-04-01), Furutani et al.
The Operon, edited by Miller & Reznikoff, Cold Spring Harbor Laboratory, 1980, pp. 42-59, 326-328, & 389-392.
Kawamura et al., Construction of a B. subtilis abl. mut. deficientin extracellular alk and neutral proteases, J. Bact. 160:442, 1984.
Yang et al., Cloning of the neutral protease gene of B. subtilis and the use of the cloned gene to create an in vitro derived dele. mutation, J. Bact. 160: 15, 1984.
Ikeda et al. "Developing of Bacillus subtilis Host-Vector Systems for Protein Production and Secretion Production of Protein A-VIP Fused Protein." Magazine of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, vol. 65, No. 03 (Mar. 1991), p. 346. (with translation).
Nakayama et al. "Efficient secretion of the authentic mature human growth hormone by Bacillus Subtilis" Journal of Biotechnology, vol. 8 (1988), pp. 123-134.
Sloma et al., J. Bacteriol., 170, (1988), pp. 5557-5563.
"Experimental Agricultural Chemistry", pp. 284-285, Asakura Shuppan (1978) with translation.
McLaughlin, et al. "Unique Features in the Ribosome Binding Site Sequence of the Gram-positive Staphylococcus aureua .beta.-Lactamase Gene" The Journal of Biological Chemistry, vol. 256, (Nov. 10, 1981), pp. 11283-11291.
Schaeffer et al. "Contribution a l'etude genetique de la sporogenese bacterienne." Comptes Rendus, vol. 251, No. 25 (Dec. 19, 1960) pp. 3125-3127, with translation.
Fuwa, Hidetsuga "A New Method For Microdetermination of Amylase Activity By the Use of Amylose As the Substrate" The Journal of Biochemistry, vol. 41, No. 5 (1954), pp. 583-603.
Hagihara et al. "Crystalline Bacterial Proteinase" The Journal of Biochemistry, vol. 45, No. 3 (1958), pp. 185-194.
Anagnostopoulos et al. "Requirements For Transformation in Bacillus subtilis" Journal of Bacteriology, vol. 81 (1961), pp. 741-746.
Marmur, J. "A Procedure for the Isolation of Deoxyribonucleic Acid for Micro-organisms" Journal of Molecular Biology, vol. 3, No. 2 (Apr. 1961), pp. 208-218.
Saito et al. "Preparation of Transforming Deoxyribonucleic Acid by Phenol Treatment" Biochemica et Biophysica Acta, vol. 72, No. 4 (Aug. 20, 1963), pp. 619-628.
Mandel et al. "Calcium-dependent Bacteriophage DNA Infection" Journal of Molecular Biology, vol. 53 (1970), pp. 159-162.
Uehara et al. "Regulation of Neutral Protease Productivity in Bacillus subtilis: Transformation of High Protease Productivity" Journal of Bacteriology, vol. 119 (1974), pp. 82-91.
Yamaguchi et al. "Isolation of Mutants Defective in .alpha.-Amylase from Bacillus subtilis: Genetic Analyses" Journal of Bacteriology, vol. 119, No. 2 (Aug. 1974), pp. 416-424.
Chemical Abstract 84:27437u of Levy et al. "Evidence of homologous relation between thermolysin and neutral protease A of Bacillus subtilis" Proceedings of the National Academy of Sciences, vol. 72, No. 11, (1975), pp. 4341-4345.
Blobel et al., "Transfer of Proteins Across Membranes" The Journal of Cell Biology, vol. 67, No. 3 (Dec. 1975), pp. 835-851.
Biochemical Information II, Boehringer Mannheim, pp. 28-30.
Lehman, I.R. "T4 DNA Polymerase" in: Method in Enzymology, vol. 29, (1978), pp. 46-57.
Maxam et al. "A New Method for Sequencing DNA" Proceedings of the National Academy of Sciences, vol. 74, No. 2 (Feb. 1977), pp. 560.564.
Gryczan et al. "Characterization of Staphylococcus aureus Plasmids Introduced by Transformation into Bacillus subtilis, Journal of Bacteriology, vol. 134, No. 1 (Apr. 1978), pp. 318-329.
Bolivar et al. "Plasmids of Escherichia coli as Cloning Vectors," in: Methods in Enzymology (1979 ed.), vol. 68, pp. 245-267.
Contente et al. "Characterization of Plasmid Transformation in Bacillus subtilis: Kinetic Properties and the Effect of DNA Conformation" Molecular & General Genetics, vol. 167 (1979), pp. 251-258.
Chang et al. "High Frequency Transformation of Bacillus subtilis Protoplasts" Molecular & General Genetics, vol. 168 (1979), pp. 111-115.
Gryczan et al. "Molecular Cloning of Heterologous Chromosomal DNA by Recombination between a Plasmid Vector and a Homologous Resident Plasmid in Bacillus subtilis," Molecular & General Genetics, vol. 177, No. 3 (1980), pp. 459-467.
Takagi, Y. "Experimental Methods for Gene Manipulation", Kodansha (1980), p. 139.
Ishikawa et al. "Enzyme Labeling with N,N'-O-Phenylenedimaleimide" in: Enzyme Immunoassay, Ishikawa et al. ed. (Tokyo, Igaku-Shoin, 1981), pp. 67-80.
Partial Certified Translation of Otsuka, Eiko, Kagaku no. Ryoiki (Field of Chemistry).
Tanpakushitsu-Kakusan-Koso, vol. 26, No. 13 (1981) pp. 2043-2046.
Palva et al. "Nucleotide sequence of the promoter and NH.sub.2 -terminal signal peptide region of the .alpha.-amylase gene from Bacillus amyloliquefaciens" Gene, vol. 15 (1981), pp. 43-51.
Goldfarb et al. "Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis" Nature, vol. 293, No. 5830 (Sep. 1981), pp. 309-311.
Williams et al. "Expression of Excherichia coli trp genes and the mouse dihydrofolate reductase gene cloned in Bacillus subtilis" Gene, vol. 16 (1981), pp. 199-206.
Maniatis, T. Molecular Cloning, (N.Y., Cold Spring Harbor Laboratory, 1982), pp. 3-5, 51, 52 and 270-274.
Debabov, V. G. "The Industrial Use of Bacilli" in: Dubnau, D. A., ed. The Molecular Biology of the Bacilli, vol. 1, (N.Y., Academic Press 1982), pp. 331, 332.
Chang et al. "Expression of Eukaryotic Genes in B. subtilis Using Signals of penP" in: Ganesan et al., ed. Molecular Cloning and Gene Regulation in Bacilli, (N.Y., Academic Press, 1982), pp. 159-169.
Moran et al. "Nucleotide Sequences that Signal the Initiation of Transcription and Translation in Bacillus subtilis" Molecular & General Genetics, vol. 186 (1982), pp. 339-446.
Takeichi et al. "Cloning of Bacillus subtilis .alpha.-Amylase Structural Gene in Plasmid pUB110" Agricultural and Biological Chemistry, vol. 47, No. 1 (Jan. 1983), pp. 159-161.
Horinouchi, S. "Expression of Information in Gram-positive Bacteria", Tanpakushitsu Kakusan-Koso, vol. 28 (1983), pp. 1468-1478.
Fuji et al. "Molecular Cloning of a Thermostable Neutral Protease Gene from Bacillus stearothermophilus in a Vector Plasmid and Its Expression in Bacillus stearothermophilus and Bacillus subtilis" Journal of Bacteriology, vol. 154, No. 2 (May 1983), pp. 831-837.
Mezes et al. "Construction of penP.DELTA.1, Bacillus licheniformis 749/C .beta.-Lactamase Lacking Site for Lipoprotein Modification" The Journal of Biological Chemistry, vol. 258, No. 18 (Sep. 25, 1983), pp. 11211-11218.
Tomioka et al. "Abstracts of Papers" Annual Meeting of the Agricultural Society of Japan, 1983, p. 33.
Honjo et al. "Cloning and expression of the gene for neutral protease of Bacillus amyloliquefaciens in Bacillus subtilis" Journal of Biotechnology, vol. 1 (1984), pp. 265-277, Chemical Abstract No. 103:82709v.
Hayashi et al. "Modification and Processing of Bacillus Licheniformis Prepenicillinase in Escherichia coli" The Journal of Biological Chemistry, vol. 259, No. 16 (Aug. 25, 1984), pp. 10448-10454.
Vasantha et al. "Genes for Alkaline Protease and Neutral Protease from Bacillus Amyloliquefaciens Co
Akaoka Akiko
Furutani Yoshio
Honjo Masaru
Kawamura Koichi
Mita Izumi
Agency of Industrial Science and Technology
Ministry of International Trade and Industry
Nolan S. L.
Schwartz Richard A.
LandOfFree
Bacillus subtilis strain whose extracellular protease activities does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bacillus subtilis strain whose extracellular protease activities, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacillus subtilis strain whose extracellular protease activities will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1860673