Bacillus subtilis mutant strain

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252300

Reexamination Certificate

active

07981659

ABSTRACT:
NovelBacillus subtilismutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived fromBacillus subtilisvia gene disruption. TheBacillus subtilismutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of theseBacillus subtilismutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.

REFERENCES:
patent: 4450235 (1984-05-01), Dean et al.
patent: 5874278 (1999-02-01), Sloma et al.
patent: 5958728 (1999-09-01), Sloma et al.
patent: 6451560 (2002-09-01), Harwood et al.
patent: 0 164 117 (1985-12-01), None
patent: 58-190390 (1983-11-01), None
patent: 61-001381 (1986-01-01), None
patent: 4-190793 (1992-07-01), None
patent: 11-509096 (1999-08-01), None
patent: 2000-210081 (2000-08-01), None
patent: 2001-503641 (2001-03-01), None
patent: 3210315 (2001-09-01), None
patent: 2001-527401 (2001-12-01), None
patent: 2002-520017 (2002-07-01), None
patent: 2004/313169 (2004-11-01), None
patent: 2005/348641 (2005-12-01), None
patent: WO 89/04866 (1989-06-01), None
patent: WO 97/03185 (1997-01-01), None
patent: WO 98-49328 (1998-11-01), None
patent: WO 03/083125 (2003-10-01), None
Morimoto, T., et al., “Enhanced Recombinant Protein Productivity by Genome Reduction inBacillus subtilis,” DNA Research 15:73-81, Oxford Univ. Press (Apr. 2008).
Ye, R. et al., “High-Level Secretory Production of Intact, Biologically Active Staphylokinase fromBacillus subtilis,” Biotechnol. Bioeng. 62:87-96, John Wiley & Sons, Inc. (Jan. 1999).
Extended European Search Report for EPO Application No. 06798311.4-2406, European Patent Office, Munich, Germany, mailed on Dec. 15, 2008.
Akamastsu, T., et al., “Incorporation of the Whole Chromosomal DNA in Protoplast Lysates into Competent Cells ofBascillus subtilis,” Biosci. Biotechnol. Biochem. 65:823-829, Japan Society for Bioscience, Biotechnology, and Agrochemistry (2001).
New Energy and Industrial Technology Development Organization, Development of Fundamental Technology for Production Process Using Biofunction (Development of Technology for Creation and Production of Host Cells) Accomplishment Report, pp. 35-72, Japan Bioindustry Association (in Japanese) (Mar. 2003).
Kunst, F., et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature 390:249-256, Nature Publishing Group (1997).
New Energy and Industrial Technology Development Organization, Development of Fundamental Technology for Production Process Using Biofunction (Development of Technology for Creation and Production of Host Cells) Accomplishment Report, Japan Bioindustry Association (Mar. 2003) (partial English Translation of document NPL2, corresponding to pp. 48, line 16 to p. 50, line 2 and p. 56, line 2 to p. 61, line 14).
Esp@cenet Database, English language abstract for JP 2004/313169 A (listed as document FP2 on the accompanying form PTO/SB/08A).
Esp@cenet Database, English language abstract for JP 2005/348641 A (listed as document FP3 on the accompanying form PTO/SB/08A).
International Search Report for International Application No. PCT/JP2006/318986, Japanese Patent Office, Japan, mailed on Dec. 26, 2006.
Written Opinion of the International Searching Authority for International Application No. PCT/JP2006/318986, mailed Dec. 26, 2006.
Written Opinion of the International Searching Authority for International Application No. PCT/JP2006/318986 (English language translation of NPL8).
Akamatsu, T. et al., “Characterization of chromosome and plasmid transformation inBacillus subtilisusing gently lysed protoplasts,” Arch Microbiol 146(4): 353-357 (Jan. 1987), Springer-Verlag, Heidelberg, Germany.
Akamatsu, T. et al., “Incorporation of the whole chromosomal DNA in protoplast lysates into competent cells ofBacillus subtilis,” Biosci Biotechnol Biochem 65(4): 823-829 (Apr. 2001), Japan Soc. Biosci, Biotech and Agrochem., Tokyo, Japan.
Hagihara, H. et al., “Novel χ-Amylase That Is Highly Resistant to Chelating Reagents and Chemical Oxidants from the AlkaliphilicBacillusIsolate KSM-K38,” Appl. Envir. Microbiol. 67: 1744-1750 (Apr. 2001), Am. Soc. Microbiology, Washington, DC.
Henrissat, B., “A classification of glycosyl hydrolases based on amino acid sequence similarities,” Biochem. J. 280: 309-316 (Dec. 1991), Portland Press, UK.
Hoch, J.A., et al., “Transformation and Transduction in Recombination-defective Mutants ofBacillus subtilis,” J. Bacteriol. 93: 1925-1937 (Jun. 1967) Am. Soc. Microbiology, Washington, DC.
Horton, R.M. et al., “Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension,” Gene 77(1): 61-68 (Apr. 1989), Elsevier Science Publishers, B.V., Amsterdam, The Netherlands.
Kobayashi, K. et al., “EssentialBacillus subtilisgenes,” Proc. Natl. Acad. Sci. USA 100: 4678-4683 (Apr. 2003), National Academy of Sciences, Washington, DC.
Kobayashi, T., et al., “Purification and properties of an alkaline protease from alkalophilicBacillussp. KSM-K16,” Appl Microbiol Biotechnol 43(3): 473-481 (Jul. 1995), Springer-Verlag, Heidelberg, Germany.
Moriya, S. et al., “Cloning of an autonomously replicating sequence (ars) from theBacillus subtilischromosome,” Mol Microbiol 6(3): 309-315 (Feb. 1992), Blackwell Scientific Publications Ltd., Oxford, UK.
Vagner, V. et al., “A vector for systematic gene inactivation inBacillus subtilis,” Microbiology 144: 3097-3104 (Nov. 1998), Soc. Gen. Microbiology, Reading, UK.
Dialog File 351, World Patents Index Accession No. 2761208, English language abstract and patent family for JP 58-190390 A, published Nov. 7, 1983.
Dialog File 351, World Patents Index Accession No. 3532683, English language abstract and patent family for JP 61-001381 A, published Jan. 7, 1986.
Dialog File 351, World Patents Index Accession No. 8025562, English language abstract and patent family for JP 11-059096 A, published Aug. 17, 1999.
Dialog File 351, World Patents Index Accession No. 5167714, English language abstract and patent family for JP 3210315 B2, published Sep. 17, 2001.
Dialog File 351, World Patents Index Accession No. 9104877, English language abstract and patent family for JP 2001-527401 A, published Dec. 25, 2001.
Dialog File 351, World Patents Index Accession No. 9875015, English language abstract and patent family for JP 2002-520017 A, published Jul. 9, 2002.
Dialog File 351, World Patents Index Accession No. 8769198, English language abstract and patent family for JP 2001-503641 A, published Mar. 21, 2001.
Dialog File 351, World Patents Index Accession No. 10283227, English language abstract and patent family for JP 2000-210081 A, published Aug. 2, 2000.
Dialog File 351, World Patents Index Accession No. 6043451, English language abstract and patent family for JP 4-190793 A, published Jul. 9, 1992.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Bacillus subtilis mutant strain does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bacillus subtilis mutant strain, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacillus subtilis mutant strain will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2681162

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.