Bacillus strains with reduced extracellular protease levels

Details Bacillus strains with reduced extracellular protease levels Bacillus strains with reduced extracellular protease levels

1985-05-13

1989-05-09

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 43525231, 435320, 435839, 935 10, 935 56, 935 42, 935 74, C12N 120, C12N 1500, C12P 2100

Patent

active

048289949

ABSTRACT:
Bacillus strains having reduced levels of extracellular protease are produced by replacing the native chromosomal DNA sequence comprising the gene for an extracellular protease, such as subtilisin, with a partially homologous DNA sequence having an inactivating DNA segment inserted therein. The strains are useful as hosts for the expression and secretion of heterologous polypeptides or proteins.

REFERENCES:
patent: 4450236 (1984-05-01), Dean et al.
patent: 4617266 (1986-10-01), Fahnestock
Wells, J. A. et al Nucleic Acids Res 11(22): 7911-7925 1983.
Dobrzhanskaya, E. O. Chem Abstr 85:89870 1976.
Shortle, D. et al Science 271:371-373 1982.
Haldenwang, W. G. et al J. Bacteriol 142(1):90-98 1980.
Chemical Abstracts, Abstract No. 226660f 101:434 (1984).
Kawamura et al., J. Bacteriol. 160(1):422-444 (1984).
3-Biochem. Genetics, Abstract No. 18528n 102:155 (1985).
Yang et al., J. Bacteriol. 160(1): 15-21 (1985).
Niaudet et al., Gene 19: 277-284 (1982).
Chemical Abstracts, Abstract No. 155686a 98: 88 (1983).
Hofemeister et al., Mol. Gen. Genet. 189: 58-68 (1983).
K. Struhl, Gene 26: 231-242 (1983).

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