Bacillus stearothermophilus DNA polymerase with proof-reading 3'

Details Bacillus stearothermophilus DNA polymerase with proof-reading 3' Bacillus stearothermophilus DNA polymerase with proof-reading 3'

1996-05-03

1998-11-10

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 4352523, 4352525, 435325, 435419, 536 232, C12N 912, C12N 1554, C12P 1934

Patent

active

058342532

ABSTRACT:
The invention relates to DNA polymerases which are capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to isolated and cloned DNA sequences derived from the Bacillus stearothermophilus thermostable DNA polymerase, as well as the expressed polymerase itself.

REFERENCES:
Riggs et al., Construction Of Single Amino Acid Substitution Mutants Of loned Bacillus Stearothermophilus DNA Polymerase I Which Lacks 3'-5' Exonuclease Activity, Biochimica et Biophysica Acta 1307 (1996), pp. 178-86.
Aliotta et al., Thermostable Bst DNA Polymerase I Lacks A 3'-5' Proofreaidng Exonuclease Activity, Genetic Analysis Biomolecular Engineering, 12 (1996), pp. 185-95.
Sanger et al., DNA Sequencing With Chain-Terminating Inhibitors, Biochemistry: Proc. Natl. Acad. Sci. USA 74 (1977), pp. 5463-5467.
Okazaki et al., Enzymatic Synthesis Of Deoxyribonucleic Acid, The Journal of Biological Chemistry, Vo. 239, No. 1, Jan. 1964, pp. 259-568.
Shengyu et al., Heat-Stable DNA Polymerase I Large Fragment Resolves Hairpin Structure In DNA Seuencing, Scientia Sinica (Series B), Vol. XXX, No. 5, May 1987, pp. 503-506.
Jacobsen et al., The N-Terminal Amino-Acid Sequences Of DNA Polymerase I From Escherichia Coli And Of The Large And The Small Fragments Obtained By A Limited Proteolysis, Eur. J. Biochem. 45 (1974), pp. 623-627.
McClary, et al., Sequencing With The Large Fragment Of DNA Polymerase I From Bacillus Stearothermophilus, DNA Sequence-J.DNA Sequencing and Mapping, Vol. 1 (1991) pp. 173-180.
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Mead et al., BST DNA Polymerase Permits Rapid Sequence Analysis From Nanogram Amounts Of Template, Biotechniques, Vol. 11, No. 1 (1991) pp. 76-84.
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Mardis et al., Automated Methods For Single-Stranded DNA Isolation And Dideoxynucleotide DNA Sequencing Reactions On A Robotic Workstation, Biotechniques, Vol. 7, No. 8 (1989) pp. 840-850.
What's New In This Catalog?, Isotherm .TM. DNA Polymerase ((rBST DNA Polymerase Large Fragment), Epicentre Technologies Corporation (1994) p. 1.
Bio Rad, Pre-Mixed Nucleotide Sequencing Kits For BST.SM. DNA Polymerase, US Bulletin, p. 1649 (Date N.A.).
Bio Rad, Fluorescent-Abeled DNA Sequencing Reactions Using BST Polymerase 92, U.S. Bulletin 1771, pp. 1-4 (Date N.A.).

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