Bacillus pumilus strain for high yield of tetramethylpyrazine

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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C435S252500, C435S252100, C435S832000

Reexamination Certificate

active

08067212

ABSTRACT:
This invention provides a high-yield bacterial strain for producing tetramethylpyrazine namedBacillus pumilusRX3-17. The strain has been deposited in China Center for Type Culture Collection on Apr. 19, 2006. The deposit number is CCTCC M 206043. The bacterial strain, isolated from soil, is rod-shaped, 1.5 μm to 3.0 μm in length and 0.6 μm to 0.7 μm in diameter. The colony color of the strain is milky-white. The strain has typical physiological and biochemical characteristics ofBacillus pumilus. The 16s rDNA sequence of this strain shares a similarity of 99% with otherBacillus pumilusstrains. This invention belongs to the domain of biotechnology. The strain can be applied to the production of tetramethylpyrazine with glucose as the substrate, solving the bottleneck of low yield in bacterial tetramethylpyrazine fermentation.

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