Bacillus circulans KsT202 produces keratan sulfate hydrolase

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 120

Patent

active

059688068

ABSTRACT:
A keratan sulfate hydrolase of an endo-.beta.-N-acetylglucosaminidase type, obtained by culturing Bacillus circulans KsT202, has reactivity on keratan polysulfate at high concentrations, an optimum pH of 4.5-6, stability at a pH of 6-7, an optimum temperature of 50-60.degree. C., thermostability at a temperature of 45.degree. C., and a molecular weight of approximately 200,000 dalton.

REFERENCES:
patent: 3857967 (1974-12-01), Kikuchi et al.
patent: 3880994 (1975-04-01), Kawaguchi et al.
patent: 4341768 (1982-07-01), Konishi et al.
patent: 4685478 (1987-08-01), Malik et al.
patent: 5061490 (1991-10-01), Paau et al.
patent: 5290695 (1994-03-01), Morikawa et al.
Buchanan et al., Eds., "Bergey's Manual of Determinative Bacteriology", 8th Edition, Williams & Wilkins Co., Baltimore, pp. 538-539, 1974.
Takegawa et al. (1991) Eur J Biochem, 202 (1). "Complete Amino Acid Sequence of Endo-.beta.-N-Acetylglucosaminidase from Flavobacterium-sp.", pp. 175-180.
Maeno et al. (1992) J. Histochem. Cytochem. 40(11). "Nature and Distribution of Mineral-binding, KeratanSulfate-containing Glycoconjugates in Rat and Rabbit Bone", pp. 1779-1788.
Watanabe et al. (1992) J. Bacteriol., 174 (2), "Structure of the Gene Encoding Chitinase D of Bacillus Circulans WI-12 and Possible Homology of the Enzyme to Other Prokaryotic Chitinases and Class III Plant Chitinases", pp. 408-414.
Watanabe et al.(1993) J. Biol. Chem. 268 (25), "Identification of Glutamic Acid 204 and Aspartic Acid 200 in Chitinase A1 of Bacillus Circulans WI-12 as Essential Residues for Chitnase Activity", pp. 18567-18572.
Nakazawa (1989) in Keratan sulphate; Chemistry, Biology and Chemical Pathology (Greiling, H .., and Scott, J.E. Eds.) "Substrate Specificity of Keratan Sulphate Degrading Enzymes (Endo-.beta.-Galactosidase, Keratanase and Keratanase II) from Microorganisms", pp. 99-110. The Biochemical Society, London.
Angata et al. (1994) Glycobiology, 4(4), "Identification Developmental Expression and Tissue Distribution of Deaminoneuraminate Hydrolase(Kdnase) Activity in Rainbow Trout", pp. 517-523.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Bacillus circulans KsT202 produces keratan sulfate hydrolase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bacillus circulans KsT202 produces keratan sulfate hydrolase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacillus circulans KsT202 produces keratan sulfate hydrolase will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2054856

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.