Bacillus cereus enterotoxin and method of use

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 711, 435834, 4242341, 4242361, 4242461, A61K 3907, C12P 2100

Patent

active

057632260

ABSTRACT:
The characterization of the protein structure of an enterotoxin to Bacillus cereus, designated hemolysin BL, is described herein. A high-yield purification scheme which allows quantitative characterization of the hemolysin BL activity is also described.

REFERENCES:
patent: 5168063 (1992-12-01), Doyle et al.
Beecher, D.J. and Macmillan, J.D., (1990) A Novel Bicomponent Hemolysin from Bacillus cereus, Infection and Immunity, 58, pp. 2220-2227.
Beecher, D.J. and Macmillan, J.D., (1991) Characterization of the Components of Hemolysin BL from Bacillus cereus, Infection and Immunity, 59, pp. 1778-1784.
Beecher, D.J. and Lee Wong, A.C., (1994) Improved Purification and Characterization of Hemolysin BL, a Hemolytic Dermonecrotic Vascular Permeability Factor from Bacillus cereus, Infection and Immunity, 62, pp. 980-986.
Bitsaev, A.R. and Ezepchuk, Y.V., (1987) The Molecular Nature of the Pathogenic Effect Induced By B. Cereus, Mol. Genet. Mikrobiol. Virusol., 7, pp. 25-31.
Blum, H., Beier, H. and Gross, H.J., (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels, Electrophoresis, 8, pp. 93-99.
Carlson, C.R., Caugant, D.A. and Kolsto, A.-B., (1994) Genotypic Diversity among Bacillus cereus and Bacillus thuringiensis Strains, Applied and Environmental Microbiology, 60, pp. 1719-1725.
Christiansson, A., (1993) Enterotoxin production in milk by Bacillus cereus: a comparison of methods for toxin detection, Neth. Milk Dairy J., 47, pp. 79-87.
Fazekas, D.S G. and Scheidegger, D., (1980) Production of Monoclonal Antibodies: Strategy and Tactics, Journal of Immunological Methods, 35, pp. 1-21.
Gilmore, M.S., Cruz-Rodz, A.L., Leimeister-Wachter, M., Kreft, J. and Goebel, W., (1989) A Bacillus cereus Cytolytic Determinant, Cereolysin AB, Which Comprises the Phospholipase C and Sphingomyelinase Genes: Nucleotide Sequence and Genetic Linkage, Journal of Bacteriology, 171, pp. 744-753.
Glatz, B.A., Spira, W.M. and Goepfert, J.M., (1974) Alteration of Vascular Permeability in Rabbits by Culture Filtrates of Bacillus cereus and Related Species, Infections and Immunity, 10, pp. 299-303.
Glatz, B.A. and Goepfert, J.M. (1977) Production of Bacillus cereus Enterotoxin in Defined Media in Fermenter-Grown Cultures, Journal of Food Protection, 40, pp. 472-474.
Granum, P.E. and Nissen, H., (1993) Sphingomyelinase in part of the `enterotoxin complex` produced by Bacillus cereus, FEMS Microbiology Letters, 110, pp. 97-100.
Granum, P.E., Brynestad, S. and Kramer, J.M., (1993) Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections, International Journal of Food Microbiology, 17, pp. 269-279.
Hames, B.D., and Rickwood, D., Gel Electrophoresis of Proteins a practical approach, IRL Press, Washington, DC.
Harlow, E., and Lane, D., (1988) Antibodies a Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. pp. 92-136.
Heinrichs, J.H., Beecher, D.J., Macmillan, J.D. and Zilinskas, B.A., (1993) Molecular Cloning and Characterization of the hblA Gene Encoding the B Component of Hemolysin BL from Bacillus cereus, Journal of Bacteriology, 175, pp. 6760-6766.
Kramer, J.M., and Gilbert, R.J., Bacillus cereus and Other Bacillus Species, Food Hygiene Laboratory, Central Public Health Library, London, England, pp. 22-70.
Matsudaira, P., (1990) Limited N-Terminal Sequence Analysis, Methods in Enzymology, 182, pp. 602-613.
Ponder, E., (1948) Hemolysis and Related Phenomena, Grune & Stratton, New York.
Stoscheck, C.M., (1990) Quantitation of Protein, Methods in Enzymology, 182, pp. 50-68.
Thompson, N.E., Ketterhagen, M.J., Bergdoll, M.S., and Schantz, E.J., (1984) Isolation and Some Properties of an Enterotoxin Produced by Bacillus cereus, Infection and Immunity, 43, pp. 887-894.
Tu, A.T., and Miller, R.A., (1992) Natural Protein Toxins Affecting Cutaneous Microvascular Permeability, J. Toxicol.--Toxin Reviews, 11, pp. 193-239.
Turnbull, P.C.B., Bacillus cereus Toxins, F. Dorner and J. Drews (ed.), Pergamon Press, Oxford, pp. 397-448.
Turnbull, P.C.B., Jorgensen, K., Kramer, J.M., Gilbert, R.J. and Parry, J.M., (1979) Severe clinical conditions associated with Bacillus cereus and the apparent involvement of exotoxins, Journal of Clinical Pathology, 32, pp. 289-293.
Turnbull, P.C.B., Kramer, J.M., Jorgensen, K., Gilbert, R.J. and Melling, J., (1979) Properties and production characteristics of vomiting, diarrheal and necrotizing toxins of Bacillus cereus, American Journal of Clinical Nutrition, 32, pp. 219-228.
Young, J.D.-E., Leong, L.G., DiNome, M.A., and Cohn, Z.A. (1986) A semiautomated Hemolysis Microassay for Membrane Lytic Proteins, Analytical Biochemistry, 154, pp. 649-654.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Bacillus cereus enterotoxin and method of use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bacillus cereus enterotoxin and method of use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacillus cereus enterotoxin and method of use will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2197892

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.