Azo dye chromogenic substrates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

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435 4, 435 18, 435 19, 435 21, 435 24, 530300, 530350, 530802, 534770, 534771, 534774, 534781, C09B 2942, C12Q 154

Patent

active

050772007

ABSTRACT:
Chromogenic substrates for the detection of hydrolyzing enzymes, processes for the preparation of these chromogenic substrates and the use of the chromogenic substrates.
The quantification of hydrolyzing enzymes for diagnostic purposes has to date been carried out by determination of the amounts of a fluorescent or highly absorbent chromogenic dyestuff liberated in the hydrolytic reaction, detection of which has in part been possible exlusively by instruments or in the neutral to alkaline pH range. After enzymatic hydrolysis, the chromogenic substrates according to the invention lead to highly sensitive and specifically measurable signals regardless of the pH range.
Azo dyestuff compounds with the general formula

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