Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1992-10-23
1997-02-11
Gibson, Sharon A.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
548236, 552 1, C07D26330
Patent
active
056022581
ABSTRACT:
The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is decreased by electromagnetic radiation. Specifically, the chelators are related to fura-2 and utilize the addition of an azide group to the 3-position of the benzofuron ring of a fura-2 type structure. Photolysis of the azide group causes the calcium ion affinity to decrease 100 to 1000 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile esters and flash-photolyzed cause large increases in intracellular free calcium ion. These chelators are used to generate controlled fast elevation of intracellular free calcium ion concentration to mimic or modulate a number of important cellular responses, especially in nerve or muscle.
REFERENCES:
patent: 4603209 (1986-07-01), Tsien et al.
patent: 4689432 (1987-08-01), Tsien et al.
patent: 4806604 (1989-02-01), Tsien et al.
patent: 5134232 (1992-07-01), Tsien et al.
patent: 5141627 (1992-08-01), Tsien et al.
Joseph P. Y. Kao et al., "Photochemically Generated Cytoslic Calcium Pulses and Their Detection by Fluo-3*", The Journal of Biological Chemistry, May 1989, vol. 264, No. 14, pp. 8179-8184.
Jack H. Kaplan, "Photochemical Manipulation of Divalent Cation Levels", Annu. Rev. Physiol., 1990, vol. 52, pp. 897-914.
Alison M. Gurney et al, "Light-Flash Physiology with Synthetic Photosensitive Compounds", Physiological Reviews, Apr. 1987, vol. 67, No. 2, pp. 583-617.
Roger Y. Tsien, "New Calcium Indicators and Buffers with High Selectivity Against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures", Journal of the American Chemical Society, 1980, vol. 19, No. 11, pp. 2396-2404.
Akawasi Minta et al., "Fluorescent Indicators for Cytosolic Calcium Based on Rhodamine and Fluorescein Chromophores*", The Journal of Biological Chemistry, May 15, 1989, vol. 264, No. 14, pp. 8171-8178.
Roger Y. Tsien et al., "Control of Cytoplasmic Calcium with Photolabile Tetracarboxylate 2-Nitroobenzhydrol Chelators", Biophysical Journal, Nov. 1986, vol. 50, pp. 843-853.
G. C. R. Ellis-Davies et al., "A New Class of Photolabile Chelators for the Rapid Release of Divalent Cations: Generation of Caged Ca and Caged Mg", Journal of Organic Chemistry, 1988, vol. 53, No. 9, pp. 1966-1969.
S. R. Adams et al., "Biologically Useful Chelators That Release Ca.sup.2+ Upon Illumination", Journal of the American Chemical Society, 1988, vol. 110, No. 10, pp. 3212-3220.
James A. McCray et al., "Properties and Uses of Photoreactive Caged Compounds", Annu. Rev. Biophys. Chem., 1989, vol. 18, pp. 239-270.
Grzegorz Grynkiewicz et al., "A New Generation of Ca.sup.2+ Indicators with Greatly Improved Fluorescence Properties", The Journal of Biological Chemistry, Mar. 25, 1985, vol. 260, No. 6, pp. 3440-3450.
Adams Stephen R.
Tsien Roger Y.
Gibson Sharon A.
Scalzo Catherine
The Regents of University of California
LandOfFree
Azide-containing chelators whose affinity for calcium ion is dec does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Azide-containing chelators whose affinity for calcium ion is dec, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Azide-containing chelators whose affinity for calcium ion is dec will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-343284