AXMI-150 delta-endotoxin gene and methods for its use

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C514S012200, C530S350000

Reexamination Certificate

active

08084416

ABSTRACT:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.

REFERENCES:
patent: 5380831 (1995-01-01), Adang et al.
patent: 5866784 (1999-02-01), Van Mellaert et al.
patent: 5908970 (1999-06-01), Van Mellaert et al.
patent: 6172281 (2001-01-01), Van Mellaert et al.
patent: 6177615 (2001-01-01), Baum
patent: 6833449 (2004-12-01), Barton et al.
patent: 7355099 (2008-04-01), Carozzi et al.
patent: 2001/0003849 (2001-06-01), Barton et al.
patent: 2004/0197916 (2004-10-01), Carozzi et al.
patent: 2008/0040827 (2008-02-01), Donovan et al.
patent: 2008/0172764 (2008-07-01), Carozzi et al.
patent: 2008/0176801 (2008-07-01), Carozzi et al.
patent: 2009/0099081 (2009-04-01), Carozzi et al.
patent: 2010/0077507 (2010-03-01), Abad et al.
patent: 2004/074462 (2004-09-01), None
patent: WO 2004/074462 (2004-09-01), None
patent: 2005/107383 (2005-11-01), None
patent: WO 2005/107383 (2005-11-01), None
Angsuthanasombat, C., et al., “Directed Mutagenesis of theBacillus thuringiensisCry11A Toxin Reveals a Crucial Role in Larvicidal Activity of Arginine-136 in Helix 4,”J. Biochem. Mol. Biol., Sep. 2001, pp. 402-407, vol. 34, No. 5.
Aronson, A.I., and Shai, Y., “WhyBacillus thuringiensisInsecticidal Toxins are so Effective: Unique Features of Their Mode of Action,”FEMS Microbiology Letters, 2001, pp. 1-8.
De Maagd, R.A., et al., “Identification ofBacillus thuringiensisDelta-Endotoxin Cry1C Domain III Amino Acid Residues Involved in Insect Specificity,”Appl. Environ. Microbiol., Oct. 1999, pp. 4369-4374, vol. 65, No. 10.
De Maagd, R.A., et al., “HowBacillus thuringiensishas Evolved Specific Toxins to Colonize the Insect World,” Trends Genet., Apr. 2001, pp. 193-199, vol. 17, No. 4.
Guo, H.H., et al., “Protein Tolerance to Random Amino Acid Change,”PNAS, Jun. 22, 2004, pp. 9205-9210, vol. 101, No. 25.
Hill, M.A. and Preiss, J., “Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase fromEscherichia coli,” Biochem. Biophys. Res. Comm., Mar. 1998, pp. 573-577, vol. 244.
Honée, G., et al., “Nucleotide Sequence of Crystal Protein Gene Isolated fromB. thuringiensissubspeciesentomocidus60.5 Coding for a Toxin Highly Active AgainstSpodopteraSpecies,”Nucleic Acids Research, May 13, 1988, p. 6240, vol. 16, No. 13.
Jenkins, J.L., et al., “Binding ofBacillus thuringiensisCrylAc Toxin toManduca sextaAminopeptidase-N Receptor is Not Directly Related to Toxicity,”FEBS Letters, 1999, pp. 373-376, vol. 462.
Kalman, et al., “Cloning of a NovelcryIC-type Gene from a Strain ofBacillus thuringiensissubsp.galleriae,” Applied and Environmental Microbiology, 1993, pp. 1131-1137, vol. 59, No. 4.
Lazar, E., et al., “Transforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities,”Mol. Cell. Biol., Mar. 1988, pp. 1247-1252, vol. 8, No. 3.
Lee, M.K., et al., “Mutations at the Argine Residues in α8 Loop ofBacillus thuringiensisδ-endotoxin CrylAc Affect Toxicity and Binding toManduca sextaandLymantria disparAminopepidase N,”FEBS Letters, 2001, pp. 108-112, vol. 497.
Masson, L., et al., “Mutagenic Analysis of a Conserved Region of Domain III in the CrylAc Toxin ofBacillus thuringiensis,” Appl. Environ. Microbiol., Jan. 2002, pp. 194-200, vol. 68, No. 1.
Rajamohan, F., et al., “Mutations at Domain II, Loop 3, ofBacillus thuringiensisCrylAa and CrylAb δ-Endotoxins Suggest Loop 3 is Involved in Initial Binding to Lepidopteran Midguts,”J. Biol. Chem., Oct. 11, 1996, pp. 25220-25226, vol. 271, No. 41.
Sanchis, V., et al., “Nucleotide Sequence and Analysis of the N-terminal Coding Region of the Spodoptera-active δ-endotoxin Gene ofBacillus thuringiensis aizawai7.29,”Mol. Microbiol., 1989, pp. 229-238, vol. 3, No. 2.
Schwartz, J.L., et al., “Single-Site Mutations in the Conserved Alternating-Arginine Region Affect Ionic Channels Formed by CrylAa, aBacillus thuringiensisToxin,”Appl. Environ. Microbiol., Oct. 1997, pp. 3978-3984, vol. 63, No. 10.
Tounsi, S., et al., “Cloning and Study of the Expression of a Novel crylla-type Gene fromBacillus thuringiensis subsp. Kurstaki,” J. Appl. Microbiol. 2003, pp. 23-28, vol. 95.
NCBI Database Report for Accession No. AAA22343, 1991.
NCBI Database Report for Accession No. AAF37224, Direct Submission on Dec. 14, 1999.
NCBI Database Report for Accession No. AAM00264, Direct Submission on Mar. 16, 2001.
NCBI Database Report for Accession No. AAN16462, Direct Submission on Aug. 22, 2002.
NCBI Database Report for Accession No. CAA65457, Direct Submission on Mar. 18, 1996.
Database UniProt Jun. 16, 2009, “SubNarne: Full=Pesticidal crystal protein cry1Bc;” XP002573546 retrieved from EBI accession No. UNIPROT: C3IAA7.
Invitation to Pay Additional Fees and Partial Search Report of PCT/US2009/069381 dated Apr. 1, 2010, (4 pages).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

AXMI-150 delta-endotoxin gene and methods for its use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with AXMI-150 delta-endotoxin gene and methods for its use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and AXMI-150 delta-endotoxin gene and methods for its use will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4313010

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.