AXMI-028 and AXMI-029, a family of novel delta-endotoxin...

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Biocides; animal or insect repellents or attractants

Reexamination Certificate

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Details

C514S012200, C435S071300, C435S069100

Reexamination Certificate

active

07811598

ABSTRACT:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

REFERENCES:
patent: 2007/0044178 (2007-02-01), Carozzi et al.
patent: 0 382 990 (1990-08-01), None
patent: WO 93/08693 (1993-05-01), None
patent: WO 2004/074462 (2004-09-01), None
Result 4, alignment of instant SEQ ID No. 4 with SEQ ID No. 4 of Payne et al., US 5,286,486, CryVIIA(b) delta endotoxin, Uniprot record No. Q45708, Uniprot database search performed on Mar. 31, 2010.
Guo, H.H., et al., “Protein Tolerance to Random Amino Acid Change,”PNAS, Jun. 22, 2004, pp. 9205-9210, vol. 101, No. 25.
Result 1, Geneseq Database Search, Alignment of SEQ ID No. 1 with SEQ ID No. 3 of Mycogen Corp. WO 93/08693 A1, Searched on Jun. 10, 2007.
Pre-OG Notice, “Examination of Patent Applications Containing Nucleotide Sequences,” (http://www.uspto.gov/seb/offices/pac/dapp/opla/preognotice/sequence02212007.pdf), printed on Jun. 13, 2007.
NCBI Database Report for Accession No. Q03749, Mar. 21, 2006 (referenced in “Novel bacillus thuringiensis insecticidal crystal protein with a silent activity against coleopteran larvae,” Appl. Environ. Microbiol. 58(8), 2536-2542.
NCBI Database Report for Accession No. Q45707, Mar. 21, 2006 (referenced in US Patent No. 5,286,486 and characterized by the database as being a “Pesticidal crystal protein cry7Ab”).
NCBI Database Report for Accession No. Q45708, Mar. 21, 2006 (referenced in US Patent No. 5,286,486 and characterized by the database as being a “Pesticidal crystal protein cry7Ab”).

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