Avirulent microbes and uses therefor: salmonella typhi

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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424 93R, 424 93D, 424 93P, 4351723, 4353201, 4352523, 43525233, 435879, 935 60, 935 62, 935 72, A61K 39112, C12N 121

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active

052944417

ABSTRACT:
This invention provides immunogenic compositions for the immunization of a vertebrate or invertebrate comprising an avirulent derivative of S. typhi. The derivatives having a mutation of the cya and/or crp and/or cdt genes. The invention also provides immunogenic compositions for the immunization of a vertebrate and invertebrate comprising an avirulent derivative of the above type which is capable of expressing a recombinant gene derived from a pathogen of said vertebrate or invertebrate individual to produce an antigen capable of inducing an immune response against said pathogen. Other embodiments of the invention include methods of preparing immunogenic compositions from these strains, and strains useful in the preparation of the immunogenic compositions, as well as methods of stimulating the immune system to respond to an immunogenic antigen of S. typhi by administration of the immunogenic composition.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss, III
patent: 4837151 (1989-06-01), Stocker
patent: 4888170 (1989-12-01), Curtiss, III
patent: 5079165 (1992-01-01), Clements et al.
Komeda et al. 1975. Molec. Gen. Genet. 142, 289-298.
Curtis III et al. 1987. Infec. and Immun. 55, 3055-3043.
Nakayama et al. 1988. Bio/Technol. 6, 693-697.
Ferrari et al. 1985. Bio/Technol. 3, 1003-1007.
Jagusztyn-Krynicka et al. 1982 J. Gen. Microbiol. 128, 1135-1145.

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