Avirulent, immunogenic flavivirus chimeras

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

Reexamination Certificate

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C536S023400, C536S023720, C536S023100, C435S236000, C435S069200, C435S069300, C435S069400, C435S235100, C424S204100, C424S205100, C424S199100, C424S185100, C424S186100, C424S202100, C514S002600

Reexamination Certificate

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07094411

ABSTRACT:
Chimeric flaviviruses that are avirulent and immunogenic are provided. The chimeric viruses are constructed to contain amino acid mutations in the nonstructural proteins of a flavivirus. Chimeric viruses containing the attenuation-mutated nonstructural genes of the virus are used as a backbone into which the structural protein genes of a second flavivirus strain are inserted. These chimeric viruses elicit pronounced immunogenicity yet lack the accompanying clinical symptoms of viral disease. The attenuated chimeric viruses are effective as immunogens or vaccines and may be combined in a pharmaceutical composition to confer simultaneous immunity against several strains of pathogenic flaviviruses.

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