Avidin dimers effective in increasing the concentration of...

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Attached to antibody or antibody fragment or immunoglobulin;...

Reexamination Certificate

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C424S001110, C424S001650, C206S223000, C206S569000

Reexamination Certificate

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07425317

ABSTRACT:
Dimers of avidin and streptavidins (diavidins) are described wherein the linker is suberate, which in turn, is bound to different functional groups (—NH2 o-COOH) of avidin. As compared to avidin, the diavidins have shown the ability to increase the amount of labelled biotin on the target, when used in an in vitropretargeting test using supported human tenascin, the biotinylated anti-tenascin monoclonal antibody (Mab-B), avidin/diavidin, and biotin-3H. The use of such diavidins is also described in cancer diagnosis and anticancer therapy based on the three-step pretargeted radioimmunotherapy procedure.

REFERENCES:
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Chinol et al, “Biochemical modifications of avidin improve pharmacokinetics and biodistribution, and reduce immunogenicity”, British Journal of Cancer, vol. 78, No. 2, Jul. 1998, pp. 189-197.
Reznik et al, “Streptavidins with intersubunit crosslinks have enhanced stability”, Nature Biotechnology, Nature Publishing, US, vol. 14, Aug. 1998, pp. 1007-1011.
Chinol et al—Biodistribution in tumour-bearing mice of Two 90Y-labelled biotins using three-step tumour targeting, Nuclear Medicine Communications, 1997, 18, pp. 176-182.
Cremonesi et al, “Three-step radioimmunotherapy with yttrium-90 biotin: dosimetry and pharmacokinetics in cancer patients”, European Journal of Nuclear Medicine, vol. 26, No. 2, Feb. 1999, pp. 110-120.
Hart et al, “HPMA copolymer-modified avidin: Immune response”, Journal of Biomaterials Science, Polymer Edition, vol. 11, No. 1, 2000, pp. 1-12.

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