Avidin-biotin conjugated emulsions as a site specific binding sy

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Ultrasound contrast agent

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424455, 424450, 424 951, 514937, 435 75, 435111, 252338, A61K 4904, A61K 966, G01N 3353, B01D 1704

Patent

active

056909071

ABSTRACT:
A method for ligand-based binding of lipid encapsulated particles to molecular epitopes on a surface in vivo or in vitro comprises sequentially administering (a) a site-specific ligand activated with a biotin activating agent; (b) an avidin activating agent; and (c) lipid encapsulated particles activated with a biotin activating agent, whereby the ligand is conjugated to the particles through an avidin-biotin interaction and the resulting conjugate is bound to the molecular epitopes on such surface. The conjugate is effective for imaging by x-ray, ultrasound, magnetic resonance or positron emission tomography. Compositions for use in ultrasonic imaging of natural or synthetic surfaces and for enhancing the acoustic reflectivity thereof are also disclosed.

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Klibanov AL et al., "Biotinylated pH-Sensitive Liposome Containers For Guided Administration of Biologically Active Substances To the Cells.", Vestn Akad Med Navk, SSSR (USSR), (8), pp. 50-54, (1990) Month Not Available.

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