Avidin-and biotin immobilized reagents and methods of use

Details Avidin-and biotin immobilized reagents and methods of use Avidin-and biotin immobilized reagents and methods of use

1989-02-24

1994-12-20

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

428403, 428407, 422 57, 436531, 436532, 436533, 436534, 436536, 436541, 5253285, 5253333, 526286, G01N 33545, G01N 33546

Patent

active

053745167

ABSTRACT:
Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

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