Avian retrovirus-bovine growth hormone DNA

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435 91, 4351723, 4353171, 435320, 435235, 4352401, 536 27, 935 13, 935 32, 935 34, 935 57, 935 60, C12P 2100, C12P 1934, C12N 1500, C12N 700

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048289876

ABSTRACT:
Recombinant DNA molecules in which an avian retroviral long terminal repeat (LTR) has been ligated to the bovine growth hormone gene are described herein. The retroviral LTR was derived from a plasmid clone of a Schmidt Rupin strain of Rous Sarcoma Virus while the bovine growth hormone (BGH) gene was derived from a lambda-bacteriophage genomic library. Using a transient eucaryotic expression assay system, the plasmid constructs were screened for their ability to direct expression and secretion of bovine growth hormone. These constructs were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone.

REFERENCES:
patent: 4405712 (1983-09-01), Vande Woude
patent: 4686098 (1982-08-01), Kopchick et al.
German et al. (1982) Proceedings National Academy Sciences, U.S.A. vol. 79, pp. 6777-6781.
Kopchick, J. et al. 1985, DNA 4:23.
Gorman, C. et al. Nov. 1982, PNAS 79:6777.
Joyner, A. et al. Mar. 1982, PNAS 79:1573.
Sompayrac, L. and K. Danna, 1981, PNAS 78:7575.
American Type Culture Collection, Catalog of Strains II 1983, 4th Edition, p. 62.
Goeddel, D. et al. Oct. 1979, Nature 281:544.
Doehmer, J. et al. 1982, PNAS 79:2268.
Woychik, R. et al. 1982, NAR 10:7197.
Gubler, U. et al., Jul. 1983, PNAS 80:4311.
Palmiter et al., Nature 300:611-615 Dec. 16, 1982.
Bates et al., Endocrinology 71:345-360 Sep. 1962.

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