Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-05-14
1989-05-09
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 91, 4351723, 4353171, 435320, 435235, 4352401, 536 27, 935 13, 935 32, 935 34, 935 57, 935 60, C12P 2100, C12P 1934, C12N 1500, C12N 700
Patent
active
048289876
ABSTRACT:
Recombinant DNA molecules in which an avian retroviral long terminal repeat (LTR) has been ligated to the bovine growth hormone gene are described herein. The retroviral LTR was derived from a plasmid clone of a Schmidt Rupin strain of Rous Sarcoma Virus while the bovine growth hormone (BGH) gene was derived from a lambda-bacteriophage genomic library. Using a transient eucaryotic expression assay system, the plasmid constructs were screened for their ability to direct expression and secretion of bovine growth hormone. These constructs were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone.
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Kopchick John J.
Leung Frederick C.
Livelli Thomas J.
Malavarca Richard H.
Chow Frank S.
Merck & Co. , Inc.
Perrella Donald J.
Pfeiffer Hesna J.
Sedman Stephanie
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