Avian mago nashi gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S320100, C435S252300, C435S325000, C536S023100, C530S350000

Reexamination Certificate

active

06951736

ABSTRACT:
The present invention relates to isolated or non-natural nucleic acids encoding at least a portion of an avianMagoprotein or a variant thereof. Nucleic acids comprising all or part of theMago nashi-encoding region and/or an untranslated region of aMago nashicDNA are used as probes for hybridizing and detecting nucleic acids encoding all or part of aMagoprotein. Recombinant cells, tissues and animals containing recombinant nucleic acids including expression vectors and encodingMago, antibodies to theMagoproteins, assays utilizing theMagopolypeptide are within the scope of the present invention. Recombinant nucleic acid molecules may contain transcription regulatory sequences, a sequence complementary to a mRNA sequence encoding aMago-related polypeptide and transcriptional control sequences functional in a recipient cell. Oligopeptides having amino acid sequences derived from the avianMagoprotein may be used to induce the formation of polyclonal or monoclonal antibodies that specifically bind to the chickenMagoprotein.

REFERENCES:
patent: 5807708 (1998-09-01), Falb et al.
“Mutations in a newly identifiedDrosophila melanogastergene,mago nashi, disrupt germ cell formation and result in the formation of mirror-image symmetrical double abdomen embryos”; R.E. Boswell, M.E. Prout, J.C. Steichen, Development 113, 373-384, 1991.
“Themago nashilocus encodes an essential product required for germ plasm assembly in Drosophila”; P.A. Newmark, R.E. Boswell, Development 120, 1303-1313, 1994.
“Posterior localization of the Drosophilia Giα protein during early embryogenesis requires a subset of the posterior group genes”; W.J. Wolfgang, M. Forte, Int. J. Dev. Biol. 39, 581-586, 1995.
“Mago nashimediates the posterior follicle cell-to-oocyte signal to organize axis formation in Drosophila”; P.A. Newmark, S.E. Mohr, L. Gong, R.E. Boswell, Development 124, 3197-3207, 1997.
“Themago nashigene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila”; D.R. Micklem, R. Dasgupta, H. Elliott, F. Gergely, C. Davidson, A. Brand, A. Gonzalez-Reyes, D. St. Johnston, Current Biology 7, 468-478, Jun. 11, 1997.
“The Mammalian Homologue ofmago nashiEncodes a Serum-Inducible Protein”; X.F. Zhao, T. Colaizzo-Anas, N.J. Nowak, T.B. Shows, R. W. Elliott, P.D. Aplan, Genomics 47, 319-322, 1998.
“Mag-1, a Homolog ofDrosophila mago nashi, Regulates Hermaphrodite Germ-Line Sex Determination inCaenorhabditis elegans”; W. Li, R. Boswell, W.B. Wood, Developmental Biology 218, 172-182, 2000.
“Magoh Interacts with a Novel RNA-Binding Protein”; X.F. Zhao, N.J. Nowak, T.B. Shows, P.D. Aplan, Genomics 63, 145-148, 2000.

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