Avian herpesvirus-based live recombinant avian vaccine, in parti

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...

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4352351, 4353201, 4242291, 4242041, A61K 39295, A61K 39255, A61K 3912, C12N 701

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active

057335540

ABSTRACT:
The present invention relates to vaccines for avian use which are based on live recombinant avian herpesviruses, in particular the Marek's disease viruses including HVT virus (herpesvirus of turkey), in which has been inserted, by genetic recombination, at least one nucleotide sequence which encodes and expresses an antigenic polypeptide of an avian pathogen under conditions which ensure immunization leading to efficient protection of the vaccinated animal against the pathogen. In one embodiment, the antigenic polypeptide is inserted in the UL43 gene under the control of the CMV immediate early promoter. The vaccines of the present invention are advantageous over previously used vaccines in that they can be used to ensure total protection of animals against Gumboro disease and to immunize chicks as young as one-day old without secondary effects, and only require low doses.

REFERENCES:
Bradley, G. et al. 1989. Journal of Virology, vol. 63, pp. 2534-2542.
"Herpesviruses As Vectors", Janis K. McMillen, Ph.D., SyntroVet Incorporated pp. 355-363. (1994).
"Protection of Chickens from Newcastle and Marek's Diseases with a Recombinant Herpesvirus of Turkeys . . . ", Morgan et al., Avian Diseases 36:858-870, 1992.
"Efficacy in Chickens of a Herpesvirus of Turkeys Recombinant Vaccine Containing the Fusion Gene . . . ", Morgan et al., Avian Diseases, 37:1032-1040, 1993.
"Influence of Dose and Route of Inoculation on Responses of Chickens to Recombinant Fowlpox Virus Vaccines", Boyle et al., Veterinary Micro-Biology 41 (1994) 173-181.
"Infection Bursal Disease Virus Structural Protein VP2 Expressed by a Fowlpox Virus Recombinant Confers Protection Against Disease in Chickens", Heine et al Arch Virol (1993) 131:277-292.
"A Recombinant Fowlpox Virus that Expresses the VP2 Antigen of Infectious Bursal Disease Virus Induces Protection Against Mortality Caused by the Virus", Bayliss et al, Arch Virol (1991) 120:193-205.
"Plasmid-Associated Effects on Test Gene Expression and Marek's Disease Virus Plaque Formation during Recombination Trials", Marshall et al, Journal of Virological Methods, 40 (1992) 195-204.
"Selection of Marek's Disease Virus Recombinants Expressing the Escherichia coli gpt Gene", Marshall et al., Virology 195, 638-648 (1993).

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