Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-04-12
2005-04-12
Leffers, Gerry (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S069100, C435S069700, C435S029000, C435S320100, C435S235100, C435S325000, C435S252300, C536S023100, C536S024100, C424S009100
Reexamination Certificate
active
06878516
ABSTRACT:
A screen has been designed to genetically engineer microbial pathogens so that expression of specific genes can be regulated in vitro and during host infection to facilitate the identification of bacterial genes essential for maintaining an infection. Specifically, gene regulatory elements which respond to the presence or absence of tetracycline are used to regulate the expression of endogenous bacterial genes. Because tetracycline is not normally present in animals, a tetracycline-regulated microbial gene can be controlled in vivo by adding or removing tetracycline from the infected animals' diet.
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Ford Charles W.
Quinn Cheryl L.
Benson Gregg C.
Leffers Gerry
Pharmacia & Upjohn Company
Reininger Irene M.
Richardson Peter C.
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