Autonomously replicating sequences, GAPDH gene and promoter deri

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 6, 435 29, 435 694, 435 695, 435 696, 435 6951, 435 6952, 4353201, 4352523, 4352541, 43525411, 4352556, 435215, 435206, 536 234, 536 237, 536 2374, 536 241, C12Q 168, C12N 1581, C12N 972, C12N 119

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059357894

ABSTRACT:
Autonomously replicating sequences(ARS), glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene and GAPDH promoter derived from Hansenula polymorpha DL-1(ATCC 26012); a vector for H. polymorpha which contains the novel ARS and is capable of inserting tandem repeating multiple copies of a polynucleotide encoding a foreign protein to the chromosome of H. polymorpha; a process for the production of a foreign protein in H. polymorpha by employing said vector; and a method for the selection of transformed H. polymorpha having multiple copies of integrated foreign genes.

REFERENCES:
Sohn, J.-H.; Choi, E.-S.; Kim, C.-H.; Agaphonov, M.O., Ter-Avanesyan, M.D.; Rhee, J.-S.; Rhee, S.-K.; A Novel Autonomously Replicating Sequence (ARS) for Multiple Integration in the Yeast Hansenula polymorpha DL-1, Journal of Bacteriology, vol. 178, No. 15, Aug. 1996, pp. 4420-4428.

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