Autonomously replicating plasmid and saccharomycopsis lipolytica

Details Autonomously replicating plasmid and saccharomycopsis lipolytica Autonomously replicating plasmid and saccharomycopsis lipolytica

1988-12-16

1992-06-02

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

4353201, C12N 119, C12N 1581

Patent

active

051186250

ABSTRACT:
The plasmid of the present invention contains Leu 2 gene (.beta.-isopropylmaleate dehydrogenase gene) from Saccharomycopsis lipolytica and an autonomously replicating sequence (hereafter referred to as ARS) and can be a shuttle vector between Escherichia coli and Saccharomycopsis lipolytica since it is produced from shuttle vector pVC 1 of Escherichia coli and Saccharomyces cerevisiae. An exogenous useful gene is inserted into the plasmid of the present invention, namely, shuttle vector to transform Escherichia coli, whereby a large quantity of plasmids are obtained. It becomes possible to produce a useful substance in Saccharomycopsis lipolytica as a host using the plasmid.

REFERENCES:
Stinchcomb et al., Nature (282) Nov. 1979 pp. 39-43.
Wing, "Development of the molecular biology of the yeast Yarrowia lipolytica: I. Development of a transformation system and search for autonomously replicating sequences. II. Cloning and sequencing of the alkaline extracellular protease structural gene.", Dissertation Abstracts International, vol. 49, No. 4, pp. 1011-B (1988).
Van der Walt et al, "The yeast genus Yarrowia gen. nov.", Antonie van Leeuwenhoek, vol. 46, pp. 517-521 (1980).
Beach et al, "Isolation of chromosomal origins of replication in yeast", Nature, vol. 284, pp. 185-187, (1980).

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