Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical
Reexamination Certificate
2007-03-06
2007-03-06
Kim, Young J. (Department: 1637)
Data processing: measuring, calibrating, or testing
Measurement system in a specific environment
Biological or biochemical
C435S006120, C435S091100, C435S091200
Reexamination Certificate
active
10155877
ABSTRACT:
Disclosed are systems and methods for identifying and quantitating the presence of one or more DNA species in a sample population through PCR amplification. DNA species quantitation includes a determination of a threshold fluorescence value used in the assessment of the PCR amplification reaction. Various embodiments of the present invention incorporate an enhancement function useful in selecting appropriate threshold fluorescence values and facilitate the determination of DNA concentrations by quantitative PCR based methodologies.
REFERENCES:
patent: 6174670 (2001-01-01), Wittwer et al.
patent: 6303305 (2001-10-01), Wittwer et al.
patent: 6387621 (2002-05-01), Wittwer
Lie et al., “Advances in quantitative PCR technology: 5′ nuclease assays,” Current Opinion in Biotechnology, 1998, vol. 9, pp. 43-48.
Woudenberg et al., “Quantitative PCR by Real Time Detection,” SPIE, 1996, vol. 2680, pp. 306-315.
“LightCycler Relative Quanitification Software”, Roche Molecular Biochemicals, Version 1.0, Mar. 2001.
“LightCycler Absolute Quanitification with External Standards”, Roche Molecular Biochemicals, Technical Note No. LC Nov. 2000.
Jensen Adrian
Lyon Justin
McLeman Cameron
Tysinger Bryan
Ward Lesley
Applera Corporation
Kilyk & Bowersox P.L.L.C.
Kim Young J.
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