Automatic threshold setting and baseline determination for...

Details Automatic threshold setting and baseline determination for... Automatic threshold setting and baseline determination for...

2007-06-05

2007-06-05

Borin, Michael (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120, C435S091200

Reexamination Certificate

active

10152280

ABSTRACT:
The invention discloses a system and methods for quantitating the presence of nucleic acid sequences by evaluation of amplification data generated using real-time PCR. In one aspect, the methods may be adapted to identify a threshold and threshold cycle for one or more reactions based upon evaluation of exponential and baseline regions for each amplification reaction. The methodology used in the analysis may be readily automated such that subjective user interpretation of the data is substantially reduced or eliminated.

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