Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-07-16
2000-12-19
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 536 243, C12Q 168, C12P 1934, C07H 2104
Patent
active
061626023
ABSTRACT:
An improved apparatus and method for nucleic acid base sequencing by annealing a primer to a nucleic acid template, extending the primer and direct step-wise detecting of incorporated bases, without the use of nucleic acid fragments, extrinsic labeling or electrophoresis, the apparatus described by employing a simple rotary valve system to generate sequential growth of a molecular chain, a laser beam and detection system for determining the amount of native fluorescence quenched in an aliquot of reaction mixture solution as each nucleotide species is extracted from a reactant solution and incorporated into the growing chain.
REFERENCES:
patent: 4863849 (1989-09-01), Melamede
patent: 5552278 (1996-09-01), Brenner
Milofsky et a. "Native fluorescence detection of Nucleic Acids and DNA restriction fragments in capillary electrophoresis" Analytical Chemistry vol. 65, pp. 153-157, 1993.
Maniatis et al. "Molecular cloning: a laboratory manual" Cold Spring Harbor Laboratory, USA, 1992.
Skoog et al. "Fundamentals of Analytical Chemistry" W.B. Saunders Company, USA, 1988.
Brusca John S.
Lundgren Jeffrey S.
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