Automatic cycling reaction apparatus and automatic analyzing app

Details Automatic cycling reaction apparatus and automatic analyzing app Automatic cycling reaction apparatus and automatic analyzing app

1985-05-15

1991-01-01

Kamen, Noah P.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435291, 422 64, 422 67, C12M 138

Patent

active

049818015

ABSTRACT:
An apparatus for carrying out an enzymatic cycling reaction including a turntable arranged in a reaction tank, a number of reaction vessels being arranged in the turntable along its periphery, a device for circulating an antifreeze liquid through the reaction tank, a heater for heating the antifreeze liquid, a refrigerator for cooling the antifreeze liquid and a switching valve for selectively passing the antifreeze liquid through the heater or refrigerator. Until all the samples and cycling mixture are delivered into the reaction vessels, the reaction tank is held at a very low temperature at which no enzymatic cycling reaction proceeds. Then the antifreeze liquid is heated to an enzymatic cycling reaction temperature so as to perform the enzymatic cycling reaction simultaneously for liquids contained in all the reaction vessels for a desired period. Then, the antifreeze liquid is heated to a high temperature so as to stop simultaneously the cycling reaction in all the reaction vessels.

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Analytical Chemistry, vol. 42, No. 6, May 1970, pp. 579-585 U.S.; E. W. Owen: "Sensitive, Wide-Range, Temperature Controlled Cell".

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