Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1990-06-22
1993-02-16
Housel, James C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435287, 435290, 435316, 935 77, 935 78, 935 85, 935 88, C12P 1934, C12M 138
Patent
active
051870843
ABSTRACT:
Apparatus and method for automatically developing, maintaining and repetitively duplicating a selectable predetermined temperature profile for replicating and amplifying a sequence of a stretch of DNA or RNA through use of a polymerase. An array of sample-containing vessels is supported in a reaction chamber through which a heat transfer medium in heat-exchange relationship with the vessels. The temperature of the air is controlled as a function of time to provide a preselectable sequence defining a temperature profile. The profile is cyclically repetitively reproduced to effect amplification of the desired sequence of DNA or RNA.
REFERENCES:
patent: 4590158 (1986-05-01), Eikman
patent: 4683202 (1987-07-01), Mullis
patent: 4950608 (1990-08-01), Kishimoto
Rolio et al., Nucleic Acids Research, 16(7), pp. 3105-3106 (1988).
Weier et al., DNA, 7(6), pp. 441-447 (1988).
Halliday & Resnick, Fundamentals of Physics 2nd Ed. Extended pp. 358-360, 1981, John Wiley & Sons.
PGC Scientifics, p. 106 Summer '90.
Berkman Michael G.
Brookens Ronald G.
Chan William
Housel James C.
The Dow Chemical Company
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