Automatic air temperature cycler and method of use in polymerose

Details Automatic air temperature cycler and method of use in polymerose Automatic air temperature cycler and method of use in polymerose

1990-06-22

1993-02-16

Housel, James C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435287, 435290, 435316, 935 77, 935 78, 935 85, 935 88, C12P 1934, C12M 138

Patent

active

051870843

ABSTRACT:
Apparatus and method for automatically developing, maintaining and repetitively duplicating a selectable predetermined temperature profile for replicating and amplifying a sequence of a stretch of DNA or RNA through use of a polymerase. An array of sample-containing vessels is supported in a reaction chamber through which a heat transfer medium in heat-exchange relationship with the vessels. The temperature of the air is controlled as a function of time to provide a preselectable sequence defining a temperature profile. The profile is cyclically repetitively reproduced to effect amplification of the desired sequence of DNA or RNA.

REFERENCES:
patent: 4590158 (1986-05-01), Eikman
patent: 4683202 (1987-07-01), Mullis
patent: 4950608 (1990-08-01), Kishimoto
Rolio et al., Nucleic Acids Research, 16(7), pp. 3105-3106 (1988).
Weier et al., DNA, 7(6), pp. 441-447 (1988).
Halliday & Resnick, Fundamentals of Physics 2nd Ed. Extended pp. 358-360, 1981, John Wiley & Sons.
PGC Scientifics, p. 106 Summer '90.

Affiliated with

Also associated with

Rating

Automatic air temperature cycler and method of use in polymerose does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Automatic air temperature cycler and method of use in polymerose, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Automatic air temperature cycler and method of use in polymerose will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2147774