Automated urinalysis method for detecting blood in urine

Details Automated urinalysis method for detecting blood in urine Automated urinalysis method for detecting blood in urine

1997-01-23

1998-03-31

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

436164, 436171, 436904, G01N 2178, G01N 33493

Patent

active

057337853

ABSTRACT:
Detecting red blood cells in a patient's urine by placing an aliquot of the urine in an automated analyzer sampling cup. The urine is transferred to a cuvette mounted within the automated analyzer and one or more reagent compositions in an aqueous medium is injected into the cuvette. The reagent contains a buffer to adjust pH, chemicals to remove substances in the urine that interfere with colorimetric photometry, a compound to lyse the red blood cells in the urine, a substrate to react with the hemoglobin, a color indicator and a surfactant to improve fluid mechanics. The sample is read against a standard using a pre-programmed monochromatically specified wavelength to determine the presence or absence of blood cells in the patient's urine.

REFERENCES:
patent: 3907503 (1975-09-01), Betts et al.
patent: 4755472 (1988-07-01), Ismail et al.
patent: 5042502 (1991-08-01), Guirguis
patent: 5089420 (1992-02-01), Albarella et al.

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