Chemical apparatus and process disinfecting – deodorizing – preser – Chemical reactor – Organic polymerization
Patent
1993-04-29
1999-08-10
Tran, Hien
Chemical apparatus and process disinfecting, deodorizing, preser
Chemical reactor
Organic polymerization
422129, C07H 2102, C07H 2104
Patent
active
059355271
ABSTRACT:
A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.
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Andrus William A.
McCollum Christie D.
Zon Gerald
Macevicz Stephen C.
Powers Vincent M.
The Perkin-Elmer Corporation
Tran Hien
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