Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1990-07-27
1993-11-16
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 253, C07H 2102, C07H 2104
Patent
active
052625303
ABSTRACT:
A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.
REFERENCES:
patent: 4373071 (1983-02-01), Itakura et al.
patent: 4379843 (1983-04-01), Cashion
patent: 4401796 (1983-08-01), Itakura et al.
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4577012 (1987-03-01), Tsurushima et al.
patent: 4689405 (1987-08-01), Frank et al.
patent: 4725677 (1988-02-01), Koster et al.
patent: 4816571 (1989-03-01), Andrus et al.
patent: 5047524 (1991-09-01), Andrus et al.
Amarnath et al. Chemical Reviews, vol. 77, pp. 183-217 (1977).
Caruthers et al. in Setlow et al., Eds., Genetic Engineering, vol. 4, pp. 1-17 (1982).
Froehler et al. Nucleic Acids Research, vol. 14, pp. 5399-5407 (1986).
Dupont Nensorb Product Bulletin, dated May 1987 and Nensorb Instruction Manual dated Jun. 8, 1987.
Pon et al. BioTechniques, vol. 6, pp. 768-775 (1988).
Gait in Hodge et al., Eds, Polymer-Supported Reactions in Organic Synthesis (John Wiley & Sons, 1980), pp. 435-456.
Sherrington, pp. 1-74 in Hodge et al., Eds. Polymer-Supported Reactions in Organic Synthesis (John Wiley & Sons, 1980).
Wallace et al., pp. 631-663 in Scouten, Ed. Solid Phase Biochemistry (John Wiley & Sons, 1982).
Koster et al. Liebigs Ann. Chem. 1974, pp. 946-958.
Ikuta et al. Anal. Chem. vol. 56, pp. 2253-2256, (1984).
Germann et al. Anal. Biochem., vol. 165, pp. 399-405 (1987).
Andrus William A.
McCollum Christie D.
Zon Gerald
Applied Biosystems Inc.
Brown Johnnie R.
Kunz Gary L.
Macevicz Stephen C.
LandOfFree
Automated system for polynucleotide synthesis and purification does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Automated system for polynucleotide synthesis and purification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Automated system for polynucleotide synthesis and purification will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-23515