Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1995-06-06
1997-04-08
Beisner, Willliam H.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
4352877, 435808, 382128, C12M 134, C12M 118
Patent
active
056187297
ABSTRACT:
A method and apparatus for estimating drug effectiveness from a drug diffusion sample are provided. The drug diffusion sample includes a plate having a medium containing a test organism and a plurality of antibiotic disks positioned on the plate in a medium. An inhibition zone surrounds each of the antibiotic disks after a prescribed incubation period. The drug diffusion sample is illuminated, and an image of the drug diffusion sample is acquired with a video camera. The image is analyzed by determining the locations of the antibiotic disks, determining the average brightness and the brightness variance of the image in a region surrounding each of the antibiotic disks, and estimating the radius of the inhibition zone surrounding each of the antibiotic disks from the average brightness and the brightness variance. The radius of the inhibition zone is indicative of drug effectiveness.
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Murray et al. "Comparison of a Highly Automated 5-h Susceptibility Testing System, the Cobas-Bact, . . . " J. Clin. Microbiol., vol. 25 (Dec. 1987), pp. 2372-2377.
Cochand Karen S.
Izraelevitz David
Beisner Willliam H.
The Analytic Sciences Corporation
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