Automated system and method for estimating antibiotic effectiven

Details Automated system and method for estimating antibiotic effectiven Automated system and method for estimating antibiotic effectiven

1994-10-17

1997-05-13

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435808, C12Q 118, C12M 122, C12M 116

Patent

active

056291698

ABSTRACT:
A method and apparatus for estimating drug effectiveness from a drug diffusion sample are provided. The drug diffusion sample includes a plate having a medium containing a test organism and a plurality of antibiotic disks positioned on the plate in a medium. An inhibition zone surrounds each of the antibiotic disks after a prescribed incubation period. The drug diffusion sample is illuminated, and an image of the drug diffusion sample is acquired with a video camera. The image is analyzed by determining the locations of the antibiotic disks, determining the average brightness and the brightness variance of the image in a region surrounding each of the antibiotic disks, and estimating the radius of the inhibition zone surrounding each of the antibiotic disks from the average brightness and the brightness variance. The radius of the inhibition zone is indicative of drug effectiveness.

REFERENCES:
G. Hejblum et al. J Clinical Microbiology vol. 31, No. 9. pp. 2396-2401 Sep., (1993).
L. Clontz, "Image Analysis Application to Agar Diffusion Assays", American Clinical Laboratory, pp. 10-11, Aug., 1992.
W. Hsia, American Clinical Laboratory, pp. 28-29, May, 1994.

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