Automated synthesis of oligonucleotides

Details Automated synthesis of oligonucleotides Automated synthesis of oligonucleotides

1993-02-11

1994-11-29

Warden, Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Chemical reactor

Organic polymerization

422116, 422111, 435287, 435289, 935 88, G05D 700, C12M 136

Patent

active

053688236

ABSTRACT:
Apparatus and method for the automated synthesis of DNA segments utilizing multiple reaction columns, all of which are open at the inlet end to the atmosphere of a reaction chamber. A movable reagent supply line outlet is positioned adjacent to the column inlet end to apply reagent to each of the columns according to an input sequence of delivery. The delivery sequence is under processor control. Reagents are removed from all columns simultaneously through the application of vacuum at the outlet end of each column. The device enables the parallel synthesis of large numbers of different oligonucleotide sequences of different lengths.

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B. D. Warner et al., "Laboratory Methods, Construction and Evaluation of an Instrument for the Automated Synthesis of Oligodeoxyribonucleotides"; Aug. 16, 1984, pp. 403, 405-406.
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