Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Reexamination Certificate
2005-03-08
2005-03-08
Ludlow, Jan M. (Department: 1743)
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
C436S086000, C436S094000, C436S161000, C436S177000, C422S067000, C422S070000, C422S105000
Reexamination Certificate
active
06864100
ABSTRACT:
The invention relates to a process and a reagent kit for obtaining clear solutions containing cell contents from biological samples in a high throughput process, comprising the steps of preparing a plurality of protein-containing solutions which contain insoluble ingredients, in separate chambers of a multi-chamber filtration unit, eliminating insoluble ingredients by filtering the solutions through the multi-chamber filtration unit with the application of a pressure differential, while preventing cross contamination between adjacent chambers by chemical and/or mechanical means, and collecting the individual filtrates separately in collecting containers, as well as a process for recovering cell contents from the clear solutions obtained. The invention further relates to the use of an apparatus for recovering clear protein-containing solutions from biological samples.
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Lubenow Helge
Ribbe Joachim
Schäfer Frank
Steinert Kerstin
Ludlow Jan M.
O'Brien David G.
Qiagen GmbH
Yankwich Leon R.
Yankwich & Associates
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