Automated plant culture proliferation system

Details Automated plant culture proliferation system Automated plant culture proliferation system

1988-07-18

1990-03-20

Jones, Larry

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

4352404, 47 4101, C12M 300

Patent

active

049101463

ABSTRACT:
An automated plant tissue proliferation system in which mature plants within a culture vessel are cut into a plurality of plugs using a die type cutter block. The cutter block is moved using a pick and place actuator to a work station where any extraneous nutrient media or plant material adhering to the sides of the cutter are removed using an indexed membrane sheet. A pair of opposed articulated wiping arms cause the membrane sheet to be wrapped around the cutter and pulled down vertically along its sides to remove the excess material. Thereafter, the cutter is indexed to deposit some fraction of the cut cells into a container having culture medium. Fluid pressure is used to eject material from the appropriate cells onto the media material. A number of additional containers are indexed into position with the cutter until all of the plugs of material are removed. the actuator and ejection system are so operated to distribute the plugs of plant material in a preselected array in a number of containers. The system provides rapid plant proliferation minimizing labor requirements and the liklihood of tissue culture contamination.

REFERENCES:
patent: 3820480 (1974-06-01), Blackmore et al.
patent: 4408549 (1983-10-01), Quarnstrom
patent: 4456683 (1984-06-01), Lintihac et al.
patent: 4481893 (1984-11-01), Quanstrom
patent: 4616578 (1986-10-01), Talbott
patent: 4827079 (1989-05-01), Evans et al.

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