Automated optical detection of tissue perfusion by microspheres

Details Automated optical detection of tissue perfusion by microspheres Automated optical detection of tissue perfusion by microspheres

1995-04-13

1997-10-28

McGraw, Vincent P.

Optics: measuring and testing

By shade or color

With color transmitting filter

2504581, 2504591, G01N 2127, G01N 2164, A06B 603

Patent

active

056822448

ABSTRACT:
A method and apparatus for optical detection and imaging of regional circulatory flow in biological tissues for research purposes. An animal or plant organ is perfused with a saline suspension of colored and/or fluorescent microspheres. The organ is excised and fixed in the form of a specimen block for mounting in a microtome or other suitable apparatus. Under automatic control of a microcomputer equipped with a frame grabber, a surface layer of the block is removed, the resulting new exposed surface of the block receives a flash of illumination from a light source, and light reflected by colored microspheres or, alternatively, light emitted by fluorescent microspheres, is detected by a CCD video camera aimed at the block. Also under microcomputer control, light filters having suitable light bandpasses are interposed between the light sources and the block, and between the block and the camera. Video signals are converted by the microcomputer into position coordinates with associated optical intensities from which regional circulatory flow is computed and displayed on a monitor. The microspheres may be segregated into batches each having a different permutation of a set of distinct colors, such that an extended sequence of interventions may be conducted using a limited number of different colors.

REFERENCES:
patent: 3649108 (1972-03-01), Ahrens et al.
patent: 4573195 (1986-02-01), de France
patent: 5230343 (1993-07-01), Guberek et al.
patent: 5292512 (1994-03-01), Schaefer et al.

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