Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1980-06-10
1982-01-19
Wiseman, Thomas
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435184, C12Q 132
Patent
active
043117910
ABSTRACT:
A diagnostic method is disclosed, based on the automated kinetic determination of LDH and MDH isoenzymes in serum, which utilizes the comparison of two rates of LDH and MDH activity at different concentrations of substrate. A step is eliminated from the procedure previously proposed, significantly improving the method. The method is based on the principle that the pure and hybrid forms of LDH and MDH isoenzymes differ in their affinities for and are inhibited to different extends by their substrate.
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Wilkinson, Diagnostic Enzymology, Edward Arnold Publishers, Ltd., London, (1976), pp. 46-54.
Bergmeyer, et al., Methods of Enzymatic Analysis, vol. 2, Academic Press Inc., N.Y. (1974), pp. 574-578, 603-612.
Cabaud, et al. "Colorimetric Measurement of Lactic Dehydrogenase Activity of Body Fluids," Am. J. Clin. Path., vol. 30, No. 3, (1958), pp. 234-236.
Howell, et al. "Lactate-to-Pyrauate or Pyrauate-to-Lactate Assay for Lactate Dehydrogenase; A Re-examination," Clin. Chem., vol. 25, No. 2, (1979), pp. 269-272.
Plagemann, et al, "The Electrophoreticlly Distinct Forms of Mammalian Lactic Dehydrogenase," J. Biol. Chem., vol. 235, No. 8, (1960), pp. 2288-2293.
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